Figure 2. Validation of experimental design and analytical robustness.

Biologcal replicates (x3) were generated for all cell lines incubated in low glucose and standard media for 24 and 48 hours. Sample and Technical replicates were analysed at the beginning, middle and end of LC-MS analysis of samples from both time points. Experimental reproducibility was confirmed with scatter plots showing Pearson Correlation values ≥ 0.97 at both 24 hours (A(i)-(ii)) and 48 hours (A(iii)-(iv)). Biological reproducibility was established with at least 70% overlap in the proteins identified in replicate samples for each control cell line at 24 (B(i)) hour and 48 hour time points (B(ii)).