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. 2017 Jan 18;8(9):14693–14707. doi: 10.18632/oncotarget.14711

Figure 3. Reduction of MiR-744 suppresses the formation of prostate xenograft tumors in vivo.

Figure 3

(A) Fluorescence microscope is used for detecting transfection efficiency for LV-anti-miR-744 transfection and the results suggested transfection efficiencies are all more than 90%. (B) and (C) Subcutaneous tumors formed in nude mice by PC3 cells stably inhibition of miR744 or control at 28 days (n = 6/group). (D) Tumor formation growth curves after transfection of indicated cells. (E) Histograms describing the mean tumor weights of each group. Mean tumor volumes are plotted. (F) H&E and immunohistochemical staining of Ki67, activated caspase-3, CD31, and CD34 in the endpoint tumors revealed that reduced Ki67-positive cells, CD31-positive cells and CD34-positive cells, and significantly increased caspase-3-positive cells in miR-744 inhibitor-overexpressing PC3 tumors. Scale bars represent 50 μm and 100 μm. Each bar represents the mean ± SD of three independent experiments. *P < 0.05.