Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Mar;87(6):2067–2071. doi: 10.1073/pnas.87.6.2067

Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes.

R Kullberg 1, J L Owens 1, P Camacho 1, G Mandel 1, P Brehm 1
PMCID: PMC53627  PMID: 2315303

Abstract

Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of alpha-, beta-, gamma-, and delta-subunit mRNAs produced two predominant channel classes with conductances of approximately 50 and approximately 12 pS, while infrequent openings of approximately 25-pS channels were also observed. Injection of alpha-, beta-, and gamma-subunit mRNAs produced a single class of approximately 12-pS AcChoR channels, which resembled the smallest conductance channels present in alpha beta gamma omega-injected oocytes. Assembly of delta-less channels may thus explain the lowest conductance AcChoR channels in alpha beta gamma delta-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.

Full text

PDF
2067

Images in this article

2068Image
on p.2068

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerbach A., Sachs F. Flickering of a nicotinic ion channel to a subconductance state. Biophys J. 1983 Apr;42(1):1–10. doi: 10.1016/S0006-3495(83)84362-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnard E. A., Miledi R., Sumikawa K. Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. Proc R Soc Lond B Biol Sci. 1982 May 22;215(1199):241–246. doi: 10.1098/rspb.1982.0040. [DOI] [PubMed] [Google Scholar]
  3. Blair L. A., Levitan E. S., Marshall J., Dionne V. E., Barnard E. A. Single subunits of the GABAA receptor form ion channels with properties of the native receptor. Science. 1988 Oct 28;242(4878):577–579. doi: 10.1126/science.2845583. [DOI] [PubMed] [Google Scholar]
  4. Boulter J., Luyten W., Evans K., Mason P., Ballivet M., Goldman D., Stengelin S., Martin G., Heinemann S., Patrick J. Isolation of a clone coding for the alpha-subunit of a mouse acetylcholine receptor. J Neurosci. 1985 Sep;5(9):2545–2552. doi: 10.1523/JNEUROSCI.05-09-02545.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buonanno A., Mudd J., Shah V., Merlie J. P. A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit. J Biol Chem. 1986 Dec 15;261(35):16451–16458. [PubMed] [Google Scholar]
  6. Colquhoun D., Sakmann B. Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscle end-plate. J Physiol. 1985 Dec;369:501–557. doi: 10.1113/jphysiol.1985.sp015912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dani J. A. Site-directed mutagenesis and single-channel currents define the ionic channel of the nicotinic acetylcholine receptor. Trends Neurosci. 1989 Apr;12(4):125–128. doi: 10.1016/0166-2236(89)90049-0. [DOI] [PubMed] [Google Scholar]
  8. Dascal N. The use of Xenopus oocytes for the study of ion channels. CRC Crit Rev Biochem. 1987;22(4):317–387. doi: 10.3109/10409238709086960. [DOI] [PubMed] [Google Scholar]
  9. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  10. Hamill O. P., Sakmann B. Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells. Nature. 1981 Dec 3;294(5840):462–464. doi: 10.1038/294462a0. [DOI] [PubMed] [Google Scholar]
  11. Henderson L. P., Lechleiter J. D., Brehm P. Single channel properties of newly synthesized acetylcholine receptors following denervation of mammalian skeletal muscle. J Gen Physiol. 1987 Jun;89(6):999–1014. doi: 10.1085/jgp.89.6.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Imoto K., Busch C., Sakmann B., Mishina M., Konno T., Nakai J., Bujo H., Mori Y., Fukuda K., Numa S. Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance. Nature. 1988 Oct 13;335(6191):645–648. doi: 10.1038/335645a0. [DOI] [PubMed] [Google Scholar]
  13. LaPolla R. J., Mayne K. M., Davidson N. Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7970–7974. doi: 10.1073/pnas.81.24.7970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Methfessel C., Witzemann V., Takahashi T., Mishina M., Numa S., Sakmann B. Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels. Pflugers Arch. 1986 Dec;407(6):577–588. doi: 10.1007/BF00582635. [DOI] [PubMed] [Google Scholar]
  15. Miledi R., Parker I., Sumikawa K. Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes. EMBO J. 1982;1(11):1307–1312. doi: 10.1002/j.1460-2075.1982.tb01315.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mishina M., Kurosaki T., Tobimatsu T., Morimoto Y., Noda M., Yamamoto T., Terao M., Lindstrom J., Takahashi T., Kuno M. Expression of functional acetylcholine receptor from cloned cDNAs. Nature. 1984 Feb 16;307(5952):604–608. doi: 10.1038/307604a0. [DOI] [PubMed] [Google Scholar]
  17. Mishina M., Takai T., Imoto K., Noda M., Takahashi T., Numa S., Methfessel C., Sakmann B. Molecular distinction between fetal and adult forms of muscle acetylcholine receptor. Nature. 1986 May 22;321(6068):406–411. doi: 10.1038/321406a0. [DOI] [PubMed] [Google Scholar]
  18. Morris C. E., Montpetit M. Multiple conductance states of the acetylcholine receptor channel complex. Can J Physiol Pharmacol. 1986 Mar;64(3):347–355. doi: 10.1139/y86-057. [DOI] [PubMed] [Google Scholar]
  19. Owens J. L., Kullberg R. In vivo development of nicotinic acetylcholine receptor channels in Xenopus myotomal muscle. J Neurosci. 1989 Mar;9(3):1018–1028. doi: 10.1523/JNEUROSCI.09-03-01018.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Owens J. L., Kullberg R. Three conductance classes of nicotinic acetylcholine receptors are expressed in developing amphibian skeletal muscle. J Neurosci. 1989 Jul;9(7):2575–2580. doi: 10.1523/JNEUROSCI.09-07-02575.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sakmann B., Methfessel C., Mishina M., Takahashi T., Takai T., Kurasaki M., Fukuda K., Numa S. Role of acetylcholine receptor subunits in gating of the channel. Nature. 1985 Dec 12;318(6046):538–543. doi: 10.1038/318538a0. [DOI] [PubMed] [Google Scholar]
  22. Sine S. M., Steinbach J. H. Activation of a nicotinic acetylcholine receptor. Biophys J. 1984 Jan;45(1):175–185. doi: 10.1016/S0006-3495(84)84146-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Takeda K., Trautmann A. A patch-clamp study of the partial agonist actions of tubocurarine on rat myotubes. J Physiol. 1984 Apr;349:353–374. doi: 10.1113/jphysiol.1984.sp015160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. White M. M., Mayne K. M., Lester H. A., Davidson N. Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4852–4856. doi: 10.1073/pnas.82.14.4852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yoshii K., Yu L., Mayne K. M., Davidson N., Lester H. A. Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes. J Gen Physiol. 1987 Oct;90(4):553–573. doi: 10.1085/jgp.90.4.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yu L., LaPolla R. J., Davidson N. Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression. Nucleic Acids Res. 1986 Apr 25;14(8):3539–3555. doi: 10.1093/nar/14.8.3539. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES