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. 2016 Nov 9;7(47):76479–76495. doi: 10.18632/oncotarget.13259

Figure 6. Calpain inhibitor treatment does not increase significantly the mortality of lymphocytes cultured in vitro.

Figure 6

PBMC were stimulated in vitro for 120 hours with immobilized anti-CD3 antibody and untreated (control), or treated with either 10 μM calpain inhibitor II (CI II), or with 10 μM calpain inhibitor IV (CI IV) as described in Materials and Methods. Bars reflect mean percentages of Annexin V- positive (early apoptotic), Annexin V and propidium iodide (PI)-positive (late apoptotic) and PI-positive (necrotic) cells among PBMC a. and among CD4+ T cells b. Standard deviations are marked. N = 12. The differences were not statistically significant.