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. 2016 Oct 20;7(47):77696–77706. doi: 10.18632/oncotarget.12773

Figure 2. Experimental model of stroma-rich neuroblastoma.

Figure 2

A. Western blot shows that SPARC secretion is higher in less tumorigenic neuroblastoma cell lines. Conditioned media from Schwann cells is shown for comparison. Schwannian stroma-rich neuroblastoma tumors were modeled by injecting nude mice with a mixture of highly tumorigenic, highly angiogenic KCNR cells which express no SPARC. Stromal component was represented by SHEP cells with modulated SPARC expression, which are anti-angiogenic, non-tumorigenic and express high levels of SPARC. B, C. Three weeks following inoculation, large tumors developed in animals injected with KCNR alone or with mixture of KCNR and shSHEP cells, which express no SPARC. In contrast, tumors in mice injected with a mixture of KCNR and control vcSHEP cells, which express normal levels of SPARC, were significantly smaller.