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. 2016 Oct 20;7(47):77707–77720. doi: 10.18632/oncotarget.12774

Figure 4. SIRT1 represses adipogenesis by blocking Wnt signaling antagonists.

Figure 4

A. Oil red O staining for triglycerides in infected C3H10T1/2 cells differentiated for 8d. B. The infected C3H10T1/2 cells were induced by using standard differentiation protocol. A: The mRNA expression of adipocyte markers (PPARγ, aP2 and adiponectin) were detected by Real-time PCR at day 8. Bars indicate SD. *P < 0.05. n = 3 per group. C. The protein expression of adipocyte markers (PPARγ, aP2 and adiponectin) were detected by immunoblotting at day 8. D. TOPflash/FOPflash was transfecred in infected C3H10T1/2 cells. The luciferase reporter activity was measured at 24h after transfection. Bars indicate SD. *P < 0.05. n = 3 per group.