Fig. 2.

LiCl affected the differentiation of NSCs in vitro. LiCl increased MAP2 expression in NSCs (a) compared to that in untreated cells (b). GFAP expression was lower in LiCl-treated NSCs (c) compared to the untreated control (d). e Statistical analysis of change in the number of MAP2⁺ and GFAP⁺ cells derived from NSCs with or without LiCl treatment. f The mRNA levels of MAP2 and Tuj1 in NSCs were increased and the level of GFAP was decreased in the presence of LiCl. Statistical difference was determined by unpaired Student’s t tests. *p < 0.05, **p < 0.01. Scale bars in b and d were 10 μm