Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Apr;87(7):2405–2408. doi: 10.1073/pnas.87.7.2405

Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.

Y C Yan 1, Y Bai 1, L F Wang 1, S Y Miao 1, S S Koide 1
PMCID: PMC53697  PMID: 1690887

Abstract

A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid residues, Arg-Lys-Arg, was found instead of Lys-Lys-Lys at the probable membrane-cytoplasmic junction that may be a unique property of sperm membrane proteins.

Full text

PDF
2405

Images in this article

2405Image
on p.2405

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedford J. M., Moore H. D., Franklin L. E. Significance of the equatorial segment of the acrosome of the spermatozoon in eutherian mammals. Exp Cell Res. 1979 Mar 1;119(1):119–126. doi: 10.1016/0014-4827(79)90341-0. [DOI] [PubMed] [Google Scholar]
  2. Blobel G. Intracellular protein topogenesis. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496–1500. doi: 10.1073/pnas.77.3.1496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glenner G. G., Wong C. W. Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun. 1984 May 16;120(3):885–890. doi: 10.1016/s0006-291x(84)80190-4. [DOI] [PubMed] [Google Scholar]
  4. Haneji T., Koide S. S. Identification of antigen in rat spermatogenic cells interacting with an anti-human sperm monoclonal antibody. Biol Reprod. 1987 Sep;37(2):467–477. doi: 10.1095/biolreprod37.2.467. [DOI] [PubMed] [Google Scholar]
  5. Hyman B. T., Van Hoesen G. W., Beyreuther K., Masters C. L. A4 amyloid protein immunoreactivity is present in Alzheimer's disease neurofibrillary tangles. Neurosci Lett. 1989 Jul 3;101(3):352–355. doi: 10.1016/0304-3940(89)90559-4. [DOI] [PubMed] [Google Scholar]
  6. Kang J., Lemaire H. G., Unterbeck A., Salbaum J. M., Masters C. L., Grzeschik K. H., Multhaup G., Beyreuther K., Müller-Hill B. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature. 1987 Feb 19;325(6106):733–736. doi: 10.1038/325733a0. [DOI] [PubMed] [Google Scholar]
  7. Kitaguchi N., Takahashi Y., Tokushima Y., Shiojiri S., Ito H. Novel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activity. Nature. 1988 Feb 11;331(6156):530–532. doi: 10.1038/331530a0. [DOI] [PubMed] [Google Scholar]
  8. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  9. Manning R. W., Reid C. M., Lampe R. A., Davis L. G. Identification in rodents and other species of an mRNA homologous to the human beta-amyloid precursor. Brain Res. 1988 Jun;427(3):293–297. doi: 10.1016/0169-328x(88)90052-6. [DOI] [PubMed] [Google Scholar]
  10. Robakis N. K., Ramakrishna N., Wolfe G., Wisniewski H. M. Molecular cloning and characterization of a cDNA encoding the cerebrovascular and the neuritic plaque amyloid peptides. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4190–4194. doi: 10.1073/pnas.84.12.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shalgi R., Phillips D. Mechanics of sperm entry in cycling hamsters. J Ultrastruct Res. 1980 May;71(2):154–161. doi: 10.1016/s0022-5320(80)90103-3. [DOI] [PubMed] [Google Scholar]
  13. Tanzi R. E., McClatchey A. I., Lamperti E. D., Villa-Komaroff L., Gusella J. F., Neve R. L. Protease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease. Nature. 1988 Feb 11;331(6156):528–530. doi: 10.1038/331528a0. [DOI] [PubMed] [Google Scholar]
  14. Yan Y. C., Wang L. F., Koide S. S. Properties of a monoclonal antibody interacting with human sperm. Arch Androl. 1987;18(3):245–254. doi: 10.3109/01485018708988490. [DOI] [PubMed] [Google Scholar]
  15. Yan Y. C., Wang L. F., Sato E., Koide S. S. Monoclonal antibody inducing human sperm agglutination. Am J Reprod Immunol. 1983 Oct-Nov;4(3):111–115. doi: 10.1111/j.1600-0897.1983.tb00263.x. [DOI] [PubMed] [Google Scholar]
  16. von Heijne G. Membrane proteins: the amino acid composition of membrane-penetrating segments. Eur J Biochem. 1981 Nov;120(2):275–278. doi: 10.1111/j.1432-1033.1981.tb05700.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES