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Identification of RNase III cleavage sites by comparison of the levels of RNA sequencing coverage between the wild-type strain (WT) and the RNase III mutant (rnc-). Read coverage across genomic locations of known cleavage sites near sucA and pnp are shown as well as the location of the known cleavage site (gray box). Novel cleavage sites were identified by looking for peaks in the ratio of reads between the rnc deletion mutant and the WT.
