ABSTRACT
Bartonella henselae is the main causative agent of cat scratch disease. In this report, we present the draft genome sequences of 12 strains of Bartonella henselae originating from the United States, Denmark, and France. These strains were isolated from cats and belonged to either 16S rRNA genotype I or 16S rRNA genotype II.
GENOME ANNOUNCEMENT
Bartonella henselae is a Gram-negative facultative intracellular bacterium of veterinary and zoonotic importance distributed worldwide. Domestic cats and other felids are the major reservoir hosts for this pathogen (1). The transmission of B. henselae from cat to cat is ensured by the cat flea Ctenocephalides felis. Ticks and biting flies have also been reported to be potential vectors (2). In humans, B. henselae is the main causative agent of cat scratch disease (CSD). This syndrome is characterized by a self-limiting regional lymphadenopathy in immunocompetent patients; however, infected immunocompromised individuals can develop severe multiple clinical manifestations, including bacillary angiomatosis, hepatic peliosis, hepatitis, endocarditis, fever, and bacteremia (3). Three distinct genotypes have been defined on the basis of their 16S rRNA sequence. Genotype I has been associated with more severe clinical manifestations than those induced by genotype II in humans. The third genotype (I/II strains with both type I and type II 16S rRNA) is the least common (4, 5). In order to more thoroughly investigate the genetic relationships between the different genotypes, we sequenced 12 feline strains from different geographical origins. These strains originated from the United States (U4 and F1), Denmark (A233, A235, A242, and A244), and France (A20, A71, A74, A76, A112, and A121). All of the strains were isolated from cats and belonged to either 16S rRNA genotype I or 16S rRNA genotype II.
Genomic DNA was extracted from one colony isolated on a blood agar plate by using the DNeasy blood and tissue kit (Qiagen), with an additional RNaseA (Roche) treatment. Libraries were prepared using the Nextera XT kit (Illumina). Whole-genome sequencing was performed using an Illumina MiSeq platform (Illumina), according to the manufacturer’s instructions. One MiSeq paired-end 300-nucleotide (nt) read MiSeq V3 chemistry run was carried out. The raw reads were trimmed (minimum length 35 bp, quality score 0.03) and assembled in CLC Genomics Workbench 7.5.1 by de novo assembly (minimum contig length 1000 bp), producing 41 to 69 contigs (Table 1). The median read depth of the assemblies ranged from 83× for isolate 71 to 216× for isolate 76 with N50 values between 82 kbp and 117 kbp (Table 1). The sequences were annotated with the National Center for Biotechnology Information (NCBI) Prokaryotic Genomes Automatic Annotation Pipeline (PGAP) at https://www.ncbi.nlm.nih.gov/genome/annotation_prok/.
TABLE 1 .
NCBI accession numbers and assembly metrics of Bartonella henselae draft genomes
| Strain | No. of contigs | Genome size (Mbp) | N50 (bp) | Median read depth | No. of coding sequences | NCBI accession no. |
|---|---|---|---|---|---|---|
| F1 | 69 | 1.86 | 90 | 177 | 1,479 | LOAI00000000 |
| A20 | 60 | 1.84 | 111 | 88 | 1,454 | LNZX00000000 |
| A71 | 65 | 1.84 | 90 | 83 | 1,463 | LOAA00000000 |
| A74 | 63 | 1.85 | 117 | 126 | 1,461 | LNZY00000000 |
| A76 | 55 | 1.86 | 113 | 216 | 1,466 | LNZZ00000000 |
| 112 | 62 | 1.84 | 84 | 165 | 1,467 | LOAB00000000 |
| U4 | 55 | 1.81 | 110 | 150 | 1,420 | LOAH00000000 |
| A121 | 52 | 1.84 | 99 | 173 | 1,458 | LOAC00000000 |
| A233 | 44 | 1.83 | 112 | 212 | 1,461 | LOAD00000000 |
| A235 | 41 | 1.82 | 102 | 118 | 1,448 | LOAE00000000 |
| A242 | 42 | 1.86 | 108 | 154 | 1,486 | LOAF00000000 |
| A244 | 41 | 1.85 | 82 | 161 | 1,470 | LOAG00000000 |
The average size of the genomes in this study was 1.84 Mb, with 1.81 Mb being the smallest genome size (isolate U4, Table 1). On average, 1,460 coding sequences were identified in the genomes (Table 1).
A detailed report on further analyses of the draft genome sequences will be released in a future publication.
Accession number(s).
The annotated draft whole-genome sequences of these Bartonella henselae strains were deposited in DDBJ/ENA/GenBank (Table 1). The versions described in this paper are the first versions.
ACKNOWLEDGMENTS
This work was supported by the Alfort National Veterinary School (ENVA) and by the French Agency for Food, Environmental and Occupational Health and Safety (Anses).
Footnotes
Citation Woudstra C, Fach P, Chomel BB, Haddad N, Boulouis H-J. 2017. Draft genome sequences of 12 feline Bartonella henselae isolates. Genome Announc 5:e00075-17. https://doi.org/10.1128/genomeA.00075-17.
REFERENCES
- 1.Breitschwerdt EB. 2014. Bartonellosis: one health perspectives for an emerging infectious disease. ILAR J 55:46–58. doi: 10.1093/ilar/ilu015. [DOI] [PubMed] [Google Scholar]
- 2.Bouhsira E, Franc M, Boulouis HJ, Jacquiet P, Raymond-Letron I, Liénard E. 2013. Assessment of persistence of Bartonella henselae in Ctenocephalides felis. Appl Environ Microbiol 79:7439–7444. doi: 10.1128/AEM.02598-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Edouard S, Raoult D. 2010. Bartonella henselae, an ubiquitous agent of proteiform zoonotic disease [in French]. Med Mal Infect 40:319–330. doi: 10.1016/j.medmal.2009.11.004. [DOI] [PubMed] [Google Scholar]
- 4.Bergmans AM, Schellekens JF, van Embden JD, Schouls LM. 1996. Predominance of two Bartonella henselae variants among cat-scratch disease patients in the Netherlands. J Clin Microbiol 34:254–260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Woestyn S, Olivé N, Bigaignon G, Avesani V, Delmée M. 2004. Study of genotypes and virB4 secretion gene of Bartonella henselae strains from patients with clinically defined cat scratch disease. J Clin Microbiol 42:1420–1427. doi: 10.1128/JCM.42.4.1420-1427.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]