Skip to main content
. 2017 Feb 12;7(4):912–925. doi: 10.7150/thno.16615

Figure 1.

Figure 1

Construction and characterization of Rluc-JEV. (A) Strategy for the construction of the infectious cDNA clone of Rluc-JEV. The Rluc gene preceded by N-terminal 34 amino acids of capsid protein (C34) of JEV is inserted at 5'UTR-C junction of JEV genome. Rluc gene fused with the FDMV-2A sequence (designated as “Rluc-2A”) is shown in red box. (B) IFA analysis of viral protein expression in BHK-21 cells transfected with in vitro transcribed genome-length RNAs of the parental JEV and Rluc-JEV. (C) Plaque morphology of JEV and Rluc-JEV in BHK-21 cells. (D) Growth curve of Rluc-JEV and the parental JEV in BHK-21 and C6/36 cells. Cells were infected with viruses at an MOI of 1, and viral titer in the culture supernatant was determined by plaque assay on BHK-21 cells. (E) Luciferase activity of Rluc-JEV in BHK-21 cells and correlation of viral titer to Rluc activity. BHK-21 cells were infected Rluc-JEV at an MOI of 0.01. Viral titers in the culture supernatant and luciferase activity in the cells at indicated time points were determined by plaque assay and luciferase assay, respectively. (F) Luciferase signals derived from virus-infected cells at MOI of 0.01, 0.1 or 1.