Fig. 4.

NG108 and $\mathrm{CHO}\text{-}{\mathrm{hM}}_1$ ${\mathrm{Ca}}^{2+}$ responses in ${\mathrm{Ca}}^{2+}$-containing outside buffer after trains of 3-ms duration IR pulses with and without intracellular RyR and ${\mathrm{IP}}_3\mathrm{R}$ blockers. (a) IR-induced changes in intracellular ${\mathrm{Ca}}^{2+}$ dynamics. The traces without antagonists are the same as in Fig. 2 and presented here for comparison. Vertical ticks above $x$-axis indicate the IR pulses train. (b) Magnification of the ${\mathrm{Ca}}^{2+}$ responses with RyR and ${\mathrm{IP}}_3\mathrm{R}$ antagonists. Error bars (black outline with gray or black/gray checked pattern fill areas) represent the SE of the mean ($n=11$ to 16).