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. Author manuscript; available in PMC: 2017 Sep 29.
Published in final edited form as: Nature. 2017 Mar 29;544(7648):53–58. doi: 10.1038/nature21693

Extended Data Fig. 1. Imaging GMPs in normal and leukemic conditions.

Extended Data Fig. 1

a, Gating strategy used to identify GMPs showing representative FACS plots with purified GMPs (purple) stained with IF markers, and a representative WT GMP (purple circle) on bone section. b, Inducible Scl-tTA::TRE-BCR/ABL (BA) and constitutive junBflox/flox::MORE-Cre (jB) mouse models of human MPN with representative GMP FACS plots. Ctrl: control; Dox: doxycycline. c, Representative IF staining showing GMPs (purple) in the BM of diseased jB mice. d, Progression of cGMP formation with disease development in BA mice at the indicated weeks (wks) post-dox withdrawal. e, Representative examples of loose pGMPs and compact cGMPs in WT and BA mice. Solid lines indicate bone surface, dotted lines cGMPs and stars pGMPs.