Table 2.
Univariate and multivariate analyses of prognostic factors in patients with ASCC.
| Parameter |
Univariate |
|
Multivariate |
|
|---|---|---|---|---|
| |
p value |
HR |
95% CI |
p value |
| Cumulative incidence of local recurrence | ||||
| Age (>/≤ 59 y) | 0.710 | |||
| Sex (female/male) | 0.004 | 0.39 | 0.16 to 0.96 | 0.040 |
| T-category (T3/4 / T1/2) | 0.010 | 1.48 | 0.59 to 3.72 | 0.404 |
| N-category (N1–3/N0) | 0.004 | 2.29 | 0.99 to 5.30 | 0.054 |
| HPV16 load (>/≤ median)* | 0.002 | 0.28 | 0.10 to 0.77 | 0.014 |
| p16 (>/≤ median)* | 0.002 | 0.32 | 0.14 to 0.77 | 0.011 |
| CD8 (>/≤ median)* | 0.007 | 0.25 | 0.07 to 0.83 | 0.023 |
| PD-1 (>/≤ median)* | 0.003 | 0.29 | 0.12 to 0.72 | 0.007 |
| PD-L1 (>/≤ median)* | 0.017 | 0.27 | 0.08 to 0.90 | 0.033 |
| FOXP3 (>/≤median)* | 0.025 | 0.44 | 0.18 to 1.05 | 0.065 |
| pCasp-8 (>/≤ median)* | 0.013 | 0.48 | 0.20 to 1.17 | 0.105 |
| Disease-free survival | ||||
| Age (>/≤ 59 y) | 0.269 | |||
| Sex (female/male) | 0.004 | 0.44 | 0.23 to 0.82 | 0.010 |
| T-category (T3/4 / T1/2) | 0.060 | |||
| N-category (N1–3/N0) | 0.010 | 1.56 | 0.84 to 2.87 | 0.156 |
| HPV16 load (>/≤ median)* | 0.001 | 0.40 | 0.20 to 0.79 | 0.009 |
| p16 (>/≤ median)* | 0.088 | |||
| CD8 (>/≤ median)* | 0.008 | 0.42 | 0.20 to 0.87 | 0.020 |
| PD-1 (>/≤ median)* | 0.007 | 0.46 | 0.25 to 0.86 | 0.014 |
| PD-L1 (>/≤ median)* | 0.063 | |||
| FOXP3 (>/≤median)* | 0.013 | 0.54 | 0.29 to 1.00 | 0.050 |
| pCasp-8 (>/≤ median)* | 0.001 | 0.49 | 0.26 to 0.94 | 0.031 |
| Overall survival | ||||
| Age (>/≤ 59 y) | 0.050 | 2.32 | 1.13 to 4.73 | 0.021 |
| Sex (female/male) | 0.002 | 0.32 | 0.15 to 0.68 | 0.003 |
| T-category (T3/4 / T1/2) | 0.012 | 1.62 | 0.81 to 3.24 | 0.171 |
| N-category (N1–3/N0) | 0.022 | 1.11 | 0.51 to 2.43 | 0.789 |
| HPV16 load (>/≤ median)* | 0.005 | 0.44 | 0.20 to 0.95 | 0.036 |
| p16 (>/≤ median)* | 0.674 | |||
| CD8 (>/≤ median)* | 0.040 | 0.52 | 0.23 to 1.19 | 0.120 |
| PD-1 (>/≤ median)* | 0.039 | 0.54 | 0.26 to 1.10 | 0.090 |
| PD-L1 (>/≤ median)* | 0.250 | |||
| FOXP3 (>/≤median)* | 0.114 | |||
| pCasp-8 (>/≤ median)* | 0.011 | 0.51 | 0.24 to 1.07 | 0.073 |
*
Due to multicollinearity for HPV16, p16, CD8, PD-1, PD-L1, FOXP3 and caspase-8, and a limited number of events, multivariate analyses were performed separately including only one molecular parameter in conjunction with sex, T- and N-category each time. Only parameters found to be significant in the univariate analysis were included in the multivariate one. HRs and Cis originate from the respective, separate multivariate analysis.
Significant p values have been marked with bold.