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. 2016 Nov 4;8(12):18773–18791. doi: 10.18632/oncotarget.13095

Figure 5.

Figure 5

(A–D) Modulation of miRNA expression after induction of apoptosis. Effects of apoptosis inducers on the expression of mature hsa-miR-96 (C), hsa-miR-147b (B) and hsa-miR-1912 (D) in SKOV3 cells. The cells were seeded in a 6-Wellplate 24 h before treatment. 48 h after treatment with 25 nM staurosporine, 25 μM etopodise or 15 ng/μl TRAIL the cells were harvested for total RNA isolation followed by qRT-PCR. (A) Basal expression levels of mature miRNAs in SKOV3 cells were normalized to relative expression in SGBS cells (relative to an internal U6 control). The expression levels of the mature miRNAs in SKOV3 and SGBS cells was measured 48 h after treatment with the apoptosis inductors staurosporine, etoposide or TRAIL (relative to an internal U6 control and to the untreated cells of the according cell line).