Figure 3. p200 CUX1 is rapidly recruited to DNA damage sites.

(A) Cells were transfected with a plasmid expressing a p200 CUX1-GFP fusion protein. DNA was damaged using 351/364 nm laser micro-irradiation and images were acquired immediately before DNA damage and periodically thereafter using the Argon laser (488 nm). See Supplementaru Movie 1. (B) The pixel intensity in the red rectangular box in Figure 3A surrounding the DNA damage was measured using Adobe Photoshop CS6. Normalized pixel intensity in region of damage was compared to undamaged region (Figure 3A; white box), called the relative CUX1-GFP signal was plotted as a function of time. (C) Cells were submitted to 337 nm laser micro-irradiation and were either fixed immediately or returned to the incubator and fixed at the desired time point followed by immunocytochemical staining.