Skip to main content
. 2016 Dec 27;8(12):19323–19341. doi: 10.18632/oncotarget.14252

Figure 1. Hsp90 secretion promotes self-renewal and expression of stem-like gene targets.

Figure 1

A. ARCaPE and DU145 prostate cancer cells were stably transduced with either a control (LacZ) plasmid or an expression construct directing the extracellular secretion of Hsp90 (eHsp90). Protein from either total cell lystates (TCL) or conditioned media was evaluated for V5-tagged eHsp90 expression. B. Percentage of spheres formed by ARCaPE-LacZ and ARCaPE-eHsp90 as defined by the total number of spheres generated divided by the number of initial wells seeded with single cells from passages 1 and 2 (P1 and P2) in 96 well ultra-low attachment plates. Following 10-12 days, productive self-renewal was assessed by observation of a minimum of 5 cells per well. C. Graphical representation of the self-renewal potential of ARCaPE, defined by the percentage of P2 spheres divided by the percentage of P1 spheres. D, E. Total RNA was isolated from ARCaPE (D) or DU145 (E) stably transduced with either the LacZ control plasmid or the eHsp90 expression plasmid, and expression of the indicated stem-like targets was assessed by qPCR. All statistics were performed using the Student's t-test. * = p<0.05, ** p<0.01.