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. 2017 Jan 3;8(12):19507–19521. doi: 10.18632/oncotarget.14470

Figure 3. MiR-24 mediates cisplatin resistance by down-modulating BimL.

Figure 3

MiR-24 downregulates BimL at mRNA and protein levels, as assessed by qRT-PCR and Western blotting in T47D cells A. On the contrary, anti-miR-24 increased in BimL expression B. Endogenous levels of BimL are higher in differentiated T47D cells compared to stem cells C. MiR-24 transfection reduced cisplatin's effect on BimL levels D. Rescue experiment with the concomitant transfection of miR-24 and BimL cDNA lacking the 3′UTR, indicated that cisplatin resistance (assessed with a Caspase-3/7 assay) was in part reverted E. BimL also reverted the effect of miR-24 on mammosphere formation F. In A, B, E and F, data are mean values ± SD from three independent experiments. In A, B and E, significance was calculated using Student's t-test.*, p<0.05; **, p<0.01; ***, p<0,001. In F, significance was calculated with ANOVA and Bonifaci correction.***, p<0,001. Western blotting analyses are from representative experiments.