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. 2017 Jan 3;8(12):19507–19521. doi: 10.18632/oncotarget.14470

Figure 4. MiR-24 levels are regulated by hypoxia.

Figure 4

A. MiR-24 levels, analyzed by qRT-PCR, were increased under hypoxic conditions in MDA-MB-231, BT-549, MCF-7 and T47D cells. B. MiR-24 overexpression upregulates mammosphere formation under hypoxia. C, D. MiR-24 up-regulates Nanog and Oct-3/4 expression in MDA-MB-231, BT-549 and T47D cells, as assessed by real time PCR. E. MiR-24 down-regulates BimL RNA in normoxic and hypoxic conditions. In A, B, C and D, data are mean values ± SD from two independent experiments. Significance was calculated using Student's t-test.*, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0,001.