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. 2017 Feb 1;8(12):19684–19698. doi: 10.18632/oncotarget.14962

Figure 2. Knockdown of PHF14 inhibited cell proliferation.

Figure 2

Cell proliferation of PHF14-transiently knocked down A549 A., CRL-5810 B. and HeLa C. cells. D. Expression of siRNA-resistant PHF14 rescued the inhibitory effect of PHF14 RNAi. Left panels: results of the MTT assay performed in control cells and different PHF14-knockdown cells. Middle panels: statistical analysis of BrdU incorporation assay. Right panels: western blot analysis showing the efficiency of PHF14 knockdown and the expression level of siRNA-resistant PHF14 in the indicated cells. β-actin was used as a loading control. NC, non-targeting control siRNA-transfected cells; 297i, PHF14-297i siRNA-transfected cells; 1958i, PHF14-1958i siRNA-transfected cells. Data are shown as the mean values from three independent experiments ± s.d. (standard deviation). * P<0.05, ** P<0.01.