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. 2017 Feb 1;8(12):19723–19737. doi: 10.18632/oncotarget.14975

Figure 6. SND1 knockdown restrains the migration and invasion of GBM cells via weakening TGFβ1 pathway activity.

Figure 6

A. Representative images (upper) and numbers (under) of the indicated migratory and invasive cells analyzed by transwell assay. The SND1-knockdown (SND1-SH) and control (SND1-HK) sub-cell lines of U87MG and U251 cells were established by infecting lentiviruses to stably mimic the silencing effect of miR-320a. B and C. Smad2, Smad4 and MMP2 mRNA levels in the indicated cells detected by qRT-PCR and normalized against GAPDH. D. Protein levels of SND1, T-Smad2, P-Smad2, Smad4 and MMP2 in the indicated U87MG sub-cell lines untreated or stimulated with TGFβ1 measured by Western blot and normalized against β-actin. All the experiments were performed at least in triplicate and the data are presented as the mean ± SD. * P<0.05, ** P<0.01, *** P<0.001. E. Schematic illustration of the molecular pathways by which miR-320a suppresses the proliferation, migration and invasion of glioma cells.