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. 2017 Feb 16;8(12):20067–20085. doi: 10.18632/oncotarget.15379

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Copyright: © 2017 Stephan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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a. Immunofluorescence analysis of the intracellular CD95 trafficking in SKW6.4 cells. Localization analysis of SFF-CD95L/CD95 (green) with early endosomal marker Rab4A (red) or trans-Golgi marker Vti1b (red), and Cathepsin D. Co-localization is indicated in yellow. b. Measurement of A-SMase activation in control or Dynasore treated (80 μM) CD95 magnetic and non-magnetic fractions. The second activation peak of A-SMase in CD95 magnetic fractions was blocked by the dynamin inhibitor Dynasore. Represented are the mean values of four independently performed assays with control cells and two independent experiments with Dynasore treated cells. (n=2) c. Time course of CD95 internalization in magnetic and non-magnetic fractions isolated from Dynasore treated SKW6.4 cells. The fractions were analyzed with anti-CD95, anti-A-SMase and anti-Cathepsin D antibodies.