Figure 4. Depletion of HDAC8 generates aneuploid eggs.

A. Representative images of euploid and aneuploid MII eggs. Chromosome spreading was performed to count the number of chromosomes. Chromosomes were counterstained with PI. Scale bar, 2.5 μm. B. The rate of aneuploidy was recorded in control and HDAC8-KD eggs. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a p < 0.05 level of significance.