Abstract
A considerable proportion of visible mutations is reported to be caused by the insertion of mobile genetic elements in Drosophila and other organisms. We estimated transposition rates of some Drosophila mobile elements by using the lines AW and JH in which spontaneous mutations have been accumulated independently for about 400 generations. Occupied sites of the mobile elements were detected by in situ hybridization on the salivary gland chromosomes sampled from 40 AW and 30 JH lines. The rates of insertion and excision of the copia and two copia-like elements, 412 and 17.6, are very low: Insertions occurred at up to 10(-3) per second chromosome per generation (17.6) and excision occurred at about 10(-5) per site per generation (copia and 412). Insertions of the I and hobo elements occurred much more frequently. These estimates are not only important for assessing the actual rate of various types of mutations but also for developing an evolutionary theory of mobile elements themselves.
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Selected References
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- Aquadro C. F., Desse S. F., Bland M. M., Langley C. H., Laurie-Ahlberg C. C. Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster. Genetics. 1986 Dec;114(4):1165–1190. doi: 10.1093/genetics/114.4.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Banerjee J., Hazra S. K., Sen S. K. EMS-induced reversion studies in the white locus of Drosophila melanogaster. Mutat Res. 1978 Jun;50(3):309–315. doi: 10.1016/0027-5107(78)90036-2. [DOI] [PubMed] [Google Scholar]
- Bingham P. M., Judd B. H. A copy of the copia transposable element is very tightly linked to the Wa allele at the white locus of D. melanogaster. Cell. 1981 Sep;25(3):705–711. doi: 10.1016/0092-8674(81)90177-x. [DOI] [PubMed] [Google Scholar]
- Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
- Biémont C., Aouar A., Arnault C. Genome reshuffling of the copia element in an inbred line of Drosophila melanogaster. Nature. 1987 Oct 22;329(6141):742–744. doi: 10.1038/329742a0. [DOI] [PubMed] [Google Scholar]
- Blackman R. K., Grimaila R., Koehler M. M., Gelbart W. M. Mobilization of hobo elements residing within the decapentaplegic gene complex: suggestion of a new hybrid dysgenesis system in Drosophila melanogaster. Cell. 1987 May 22;49(4):497–505. doi: 10.1016/0092-8674(87)90452-1. [DOI] [PubMed] [Google Scholar]
- Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
- Carbonare B. D., Gehring W. J. Excision of copia element in a revertant of the white-apricot mutation of Drosophila melanogaster leaves behind one long-terminal repeat. Mol Gen Genet. 1985;199(1):1–6. doi: 10.1007/BF00327501. [DOI] [PubMed] [Google Scholar]
- Charlesworth B., Lapid A. A study of ten families of transposable elements on X chromosomes from a population of Drosophila melanogaster. Genet Res. 1989 Oct;54(2):113–125. doi: 10.1017/s0016672300028482. [DOI] [PubMed] [Google Scholar]
- Eggleston W. B., Johnson-Schlitz D. M., Engels W. R. P-M hybrid dysgenesis does not mobilize other transposable element families in D. melanogaster. Nature. 1988 Jan 28;331(6154):368–370. doi: 10.1038/331368a0. [DOI] [PubMed] [Google Scholar]
- Fawcett D. H., Lister C. K., Kellett E., Finnegan D. J. Transposable elements controlling I-R hybrid dysgenesis in D. melanogaster are similar to mammalian LINEs. Cell. 1986 Dec 26;47(6):1007–1015. doi: 10.1016/0092-8674(86)90815-9. [DOI] [PubMed] [Google Scholar]
- Finnegan D. J., Rubin G. M., Young M. W., Hogness D. S. Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1053–1063. doi: 10.1101/sqb.1978.042.01.106. [DOI] [PubMed] [Google Scholar]
- Gehring W. J., Paro R. Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster. Cell. 1980 Apr;19(4):897–904. doi: 10.1016/0092-8674(80)90081-1. [DOI] [PubMed] [Google Scholar]
- Goldberg M. L., Paro R., Gehring W. J. Molecular cloning of the white locus region of Drosophila melanogaster using a large transposable element. EMBO J. 1982;1(1):93–98. doi: 10.1002/j.1460-2075.1982.tb01130.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green M. M. Transposable elements in Drosophila and other Diptera. Annu Rev Genet. 1980;14:109–120. doi: 10.1146/annurev.ge.14.120180.000545. [DOI] [PubMed] [Google Scholar]
- Kidwell M. G. Evolution of hybrid dysgenesis determinants in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1655–1659. doi: 10.1073/pnas.80.6.1655. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lim J. K. Intrachromosomal rearrangements mediated by hobo transposons in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9153–9157. doi: 10.1073/pnas.85.23.9153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUKAI T. THE GENETIC STRUCTURE OF NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER. I. SPONTANEOUS MUTATION RATE OF POLYGENES CONTROLLING VIABILITY. Genetics. 1964 Jul;50:1–19. doi: 10.1093/genetics/50.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukai T., Chigusa S. I., Mettler L. E., Crow J. F. Mutation rate and dominance of genes affecting viability in Drosophila melanogaster. Genetics. 1972 Oct;72(2):335–355. doi: 10.1093/genetics/72.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukai T., Cockerham C. C. Spontaneous mutation rates at enzyme loci in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2514–2517. doi: 10.1073/pnas.74.6.2514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukai T., Harada K., Yoshimaru H. Spontaneous mutations modifying the activity of alcohol dehydrogenase (ADH) in Drosophila melanogaster. Genetics. 1984 Jan;106(1):73–84. doi: 10.1093/genetics/106.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Roeder G. S., Farabaugh P. J., Chaleff D. T., Fink G. R. The origins of gene instability in yeast. Science. 1980 Sep 19;209(4463):1375–1380. doi: 10.1126/science.6251544. [DOI] [PubMed] [Google Scholar]
- Roeder G. S., Fink G. R. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. doi: 10.1016/0092-8674(80)90131-2. [DOI] [PubMed] [Google Scholar]
- Roeder G. S., Fink G. R. Movement of yeast transposable elements by gene conversion. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5621–5625. doi: 10.1073/pnas.79.18.5621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saigo K., Millstein L., Thomas C. A., Jr The organization of Drosophila melanogaster histone genes. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):815–827. doi: 10.1101/sqb.1981.045.01.100. [DOI] [PubMed] [Google Scholar]
- Scobie N. N., Schaffer H. E. A mutator factor in a strain of Drosophila melanogaster: identified by use of mutation, reversion rates and male recombination. Genetics. 1982 Jul-Aug;101(3-4):417–429. doi: 10.1093/genetics/101.3-4.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scobie N. N., Schaffer H. E. The location of a mutator factor in a strain of Drosophila melanogaster by assaying male recombination. Genetics. 1982 Jul-Aug;101(3-4):405–416. doi: 10.1093/genetics/101.3-4.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spradling A. C., Rubin G. M. Drosophila genome organization: conserved and dynamic aspects. Annu Rev Genet. 1981;15:219–264. doi: 10.1146/annurev.ge.15.120181.001251. [DOI] [PubMed] [Google Scholar]
- Streck R. D., Macgaffey J. E., Beckendorf S. K. The structure of hobo transposable elements and their insertion sites. EMBO J. 1986 Dec 20;5(13):3615–3623. doi: 10.1002/j.1460-2075.1986.tb04690.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodruff R. C., Blount J. L., Thompson J. N., Jr Hybrid dysgenesis in D. melanogaster is not a general release mechanism for DNA transpositions. Science. 1987 Sep 4;237(4819):1206–1218. doi: 10.1126/science.2820057. [DOI] [PubMed] [Google Scholar]
- Yamaguchi O., Mukai T. Variation of spontaneous occurrence rates of chromosomal aberrations in the second chromosomes of Drosophila melanogaster. Genetics. 1974 Dec;78(4):1209–1221. doi: 10.1093/genetics/78.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yannopoulos G., Stamatis N., Monastirioti M., Hatzopoulos P., Louis C. hobo is responsible for the induction of hybrid dysgenesis by strains of Drosophila melanogaster bearing the male recombination factor 23.5MRF. Cell. 1987 May 22;49(4):487–495. doi: 10.1016/0092-8674(87)90451-x. [DOI] [PubMed] [Google Scholar]
- Zachar Z., Davison D., Garza D., Bingham P. M. A detailed developmental and structural study of the transcriptional effects of insertion of the Copia transposon into the white locus of Drosophila melanogaster. Genetics. 1985 Nov;111(3):495–515. doi: 10.1093/genetics/111.3.495. [DOI] [PMC free article] [PubMed] [Google Scholar]