Fig. 1.

ELISpot responses in paired fresh and cryopreserved cells at (a) day 63 and (b) C-1 (day 76 −4/+3). Red bars and boxes represent mean and 5th–95th CI respectively. Loss of IFNγ production in cryopreserved samples is significant after stimulation with the malaria specific antigens TRAP and CSP. Functional loss is most notable at the peak time point post MVA boost. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)