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. 2017 Mar 9;17:98–100. doi: 10.1016/j.nmni.2017.02.009

Reappearance of buffalo-origin-like porcine circovirus type 2 strains in swine herds in southern China

S-L Zhai 1,∗,4, X Zhou 1,2,4, T Lin 3,4, H Zhang 1,2, X-H Wen 1, X-R Zhou 1, C-L Jia 1, D Tu 1, X-L Zhu 1, Q-L Chen 1, W-K Wei 1, D-H Lv 1,∗
PMCID: PMC5388935  PMID: 28417007

Abstract

Previously, we identified three porcine circovirus type 2 (PCV2) strains in buffalo meat samples from southern China. In this study, we confirmed the reappearance of those buffalo-origin-like PCV2 strains in swine herds in this region, which supported the possible cross-species infection of PCV2 between buffalos and pigs under field conditions.

Keywords: Buffalo-origin, cross-species infection, porcine circovirus type 2, southern China, swine-origin

Porcine circovirus type 2 (PCV2) is an important virus for the worldwide swine industry, because it can cause a variety of clinical diseases including postweaning multisystemic wasting syndrome, porcine dermatitis and nephropathy syndrome, porcine respiratory disease complex, proliferative and necrotizing pneumonia and reproductive disorders [1]. However, in recent years, it was confirmed that PCV2 widely existed in non-porcine animals including cattle, rodents, insects and even humans [1]. Although PCV2 was reported in some diseased animals [2], [3], [4], the relationship between PCV2 and the disease in non-porcine animals was unclear. However, one animal experimental study demonstrated that calves were susceptible to PCV2 and displayed clinical signs including lymph node swelling, reddening of oral and ocular mucosa and diarrhoea [5]. Moreover, another study suggested that PCV2 might be closely related to the death of minks clinically [6], indicating that PCV2 is also an important pathogen in minks, not just in pigs.

In our previous study, we identified three different PCV2 strains (Buffalo1, Buffalo2 and Buffalo3) in buffalo meat samples bought from one fresh meat market of southern China. Genetic analysis showed that they belonged to three different PCV2 genotypes [7]. During the epidemiological survey of 2016, we obtained two significantly different PCV2 sequences (designated GDAH16 and GDSE16) from lung samples of dead pigs with respiratory disease according to a previously described method [7], [8]. GDAH16 was 1768 nucleotides in length and had two major open reading fragments (ORFs). ORF1 and ORF2 were targeted at position 51→995 and 1735→1034, and encoded the predicted replicase and capsid proteins, respectively. Online BLAST alignment analysis showed that GDAH16 shared 99.72% nucleotide similarity (only five nucleotides different) with Buffalo3 (GenBank Accession no. KM116515). However, GDSE16 was 1767 nucleotides long. Its ORF1-targeted position was the same as GDAH16, but its ORF2-targeted position (1734→1033) was different. Similarly, online BLAST alignment analysis revealed 99.55% nucleotide identity (only eight nucleotides different) between GDSE16 and Buffalo1 (GenBank Accession no. KM116513). Further alignment analysis suggested that GDAH16 and GDSE16 only had 95.6% nucleotide similarity (about 78 nucleotides different). In addition, phylogenetic analysis indicated that they were clustered in different branches (PCV2a and PCV2b) and had close genetic relationships with Buffalo-origin PCV2 isolates (Buffalo3 and Buffalo1) (GenBank Accession nos. KM116515 and KM116513), respectively (Fig. 1).

Fig. 1.

Fig. 1

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Phylogenetic analysis of porcine circovirus type 2 (PCV2). The phylogenetic tree based on open reading frame 2 (ORF2) gene sequences of PCV2 was constructed by the neighbour-joining method using MEGA 5.1 software. One thousand bootstrap replications and 0.035 distance scale length were used. Values <50% were hidden in the phylogenetic tree. The strains obtained in this study, GDAH16 and GDSE16, are indicated by the left arrows. Different origins of PCV2 are labelled by different symbols.

Possible cross-species transmission of PCV2 is reported in several previous studies [6], [8], [9], [10], [11], [12], [13], [14], which included pig–rodent transmission, pig–mink transmission, pig–human transmission and pig–insect transmission. In this study, we confirmed those buffalo-origin-like PCV2 strains in swine herds in southern China, which further supported the possible cross-species infection of PCV2 between buffalo and pig in this region.

Accession number(s)

The genome sequences of GDAH16 and GDSE16 have been deposited in GenBank under the Accession numbers KY347898, KY347899.

Conflict of interest

There is no conflict of interest.

Acknowledgements

This work was supported by Ministry of Science and Technology of the People's Republic of China (Grant no. 2015GA780010), Guangdong Provincial Department of Science and Technology (Grant nos. 2015A020208008, 2016A040403083 and 2016B020234006), and Guangzhou Science Technology and Innovation Commission (Grant no. 201508020055).

Contributor Information

S.-L. Zhai, Email: zhaishaolun@163.com.

D.-H. Lv, Email: lvdianhong@gdaas.cn.

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