Figure 9. Loss of jumu in the entire lymph gland induces the generation of lamellocytes through the activation of Toll signaling.
(A–D) Immunostaining for L1 (red) and Hnt (red) shows that the knock down of jumu in the entire lymph gland increases the number of lamellocytes (B) and reduces the number of crystal cells (D). (E) Quantification of the number of PSC cells (Antp+ cells). The data are presented in a box-and-whisker representation. ***p<0.001 (Student’s t test). (F–K) Immunostaining for L1 (red) shows that only less than 20% of lymph glands of the Srp-Gal4 control display a few lamellocytes (n = 24) (F), but numerous lamellocytes are generated in more than 60% of the lymph glands of Srp>jumu RNAi (n = 36) (G) and in more than 80% of the lymph glands of Srp>Toll10b (n = 30) (H) and Srp>UAS-col (n = 26) (I). The deficiency of dif and col in Srp>jumu RNAi clearly reduces the number of lamellocytes, and only less than 10% (n = 36) (J) and 20% of the lymph glands (n = 32) (K) generated a few lamellocytes, respectively. (L–Q) Immunostaining for Dorsal (green) shows that Dorsal is enriched in nuclei throughout the lymph glands of the control animals (L), and the expression and localization of Dorsal is not changed in the crosses (M–Q). (R–W) Immunostaining for Dif (green) shows that Dif is expressed in the cytoplasm of cells throughout the lymph glands of the control animals (R) but shows nuclear enrichment mainly in the CZ and partly in the MZ of Srp>jumu RNAi (S), Srp>Toll10b (T) and Srp>UAS-col (U). The deficiency of dif (V) and col (W) in Srp>jumu RNAi clearly reduces the nuclear enrichment of Dif. The regions indicated by the arrows are magnified. Dashed white lines outline the edges of the primary lobes. Scale bars: 100 μm.
Figure 9—figure supplement 1. jumu knockdown or col overexpression in different specific zones of the lymph gland does not affect lamellocyte differentiation.
Figure 9—figure supplement 2. Analysis of subcellular localization of Dorsal and Dif in circulating cells and the lymph gland.
