Table 2. Increased next-generation sequencing sensitivity with the addition of BNA/LNA oligonucleotides.
| High-Sensitivity NGS | Conventional NGS | |||||
|---|---|---|---|---|---|---|
| Patient # 4 | ||||||
| Gene | Nucleotide | Amino Acid | Alternate Variant Frequency | Read Depth | Alternate Variant Frequency | Read Depth |
| PLCγ2 | 1993C>T | Arg665Trp | 16.6 | 353 | 9.6 | 912 |
| PLCγ2 | 2535A>T | Leu845Phe | 62.7 | 126 | 14 | 700 |
| BTK | 1442G>C | Cys481Ser | 5 | 309 | 4 | 681 |
| BTK | 1441T>A | Cys481Ser | 6 | 310 | 7 | 680 |
| BTK | 1441T>C | Cys481Arg | 14.9 | 307 | 6 | 680 |
| Patient # 16 | ||||||
| Gene | Nucleotide | Amino Acid | Alternate Variant Frequency | Read Depth | ||
| BTK | 1442G>C | Cys481Ser | 51.2 | 697 | ||
| Patient # 5 | ||||||
| Gene | Nucleotide | Amino Acid | Alternate Variant Frequency | Read Depth | ||
| BTK | 1442G>C | Cys481Ser | 3 | 398 | ||
| Patient # 15 | ||||||
| Gene | Nucleotide | Amino Acid | Alternate Variant Frequency | Read Depth | ||
| BTK | 1442G>C | Cys481Ser | 2 | 89 | ||
| Patient # 3 | ||||||
| Gene | Nucleotide | Amino Acid | Alternate Variant Frequency | Read Depth | ||
| BTK | 1442G>C | Cys481Ser | 73.2 | 102 | ||
| PLCγ2 | 2535A>T | Leu845Phe | 3 | 145 | ||
| PLCγ2 | 2535A>C | Leu845Phe | 5 | 145 | ||
All samples used were from patients with suspected progression. High-sensitivity NGS includes BNA/LNA oligonucleotides in library preparation and conventional NGS does not.