Table 1.

Selected features

Metabolite	Ion mode	ID level	Calibration set						Validation set			Regulation
			m/z	rt	CV(QC)	Fold	VIP	p	Fold	VIP	pc
α-Hydroxybutyrate	Neg	1	103.0403	94	4	1.23	1.41	0.00001	1.35	2.67	0.00468	↑
2-Hydroxy-3-methylbutyrate	Neg	1	117.0557	206	3	1.10	0.82	0.03013	1.01	0.26	0.49282	↑
Acylcarnitine	Pos	3	356.2796	415	8	0.88	n.a.b	0.04638	0.95	0.51	0.31398	↓
Adenosine	Pos	1	268.1045	132	3	0.69	1.24	0.05063	0.83	1.76	0.01896	↓
Adenosine	Neg	1	266.0891	129	3	0.69	1.13	0.06785	0.82	2.16	0.02949	↓
Decenoylcarnitine (C10:1)e	Pos	2	314.2326	382	6	0.78	0.89	0.01355	0.75	2.12	0.02433	↓
Decenoylcarnitine (C10:1)e	Pos	2	314.2327	390	11	0.82	0.96	0.03235	0.78	2.21	0.01782	↓
Dodecenoylcarnitine (C12:1)	Pos	2	342.2640	408	20	0.80	1.01	0.02076	0.80	1.92	0.02481	↓
Decanoylcarnitine (C10)	Pos	1	316.2490	398	4	0.80	1.00	0.04788	0.80	1.78	0.03529	↓
Dimethylnonanoylcarnitine (C11)d	Pos	2	330.2640	404	12	0.80	0.96	0.02821	0.91	1.42	0.11149	↓
Dodecanoylcarnitine (C12)	Pos	1	344.2800	416	4	0.76	0.98	0.03012	0.83	1.46	0.04310	↓
Hypoxanthine	Pos	1	137.0458	48	4	0.80	1.01	0.10417	0.80	1.93	0.03704	↓
Dipeptide (Leu/Ile)f	Pos	2	245.1859	246	8	0.77	1.45	0.10772	0.92	0.93	0.26577	↓
Pantothenate	Pos	1	220.1177	201	7	1.09a	n.a.b	0.02044	1.06	0.53	0.33357	↑
Propionylcarnitine (C3)	Pos	1	218.1387	121	4	0.93a	n.a.b	0.04318	0.92	0.60	0.05926	↓
Sphinganine 1-phosphate	Pos	1	382.2721	450	4	0.80	1.10	0.22922	0.90	1.45	0.21910	↓
Sphingosine 1-phosphate	Pos	1	380.2567	442	2	0.87	1.06	0.28048	0.92	1.15	0.22508	↓
Succinyladenosine	Neg	1	382.0999	201	11	1.13	n.a.b	0.02228	1.03	0.24	0.66725	↑
Tetradecadienoylcarnitine (C14:2)e	Pos	2	368.2797	414	8	0.78	0.87	0.03150	0.75	1.85	0.01766	↓
Tetradecenoylcarnitine (C14:1)e	Pos	2	370.2957	424	4	0.80	1.02	0.03436	0.76	1.60	0.01148	↓
UMP	Neg	1	323.0282	32	8	0.77	1.08	0.19633	0.83	1.64	0.19899	↓

The ionization mode, level of identification (ID), mass to charge ratio (m/z), retention time (RT/s), coefficient of variation in quality control samples [CV(QC)], fold change, VIP score and p value for the two datasets are listed. Arrows indicate the direction of regulation when comparing plasma collected after the RIC intervention to plasma collected before

^aPantothenate and propionylcarnitine have been included due to biochemical relevance even though they are slightly less regulated than the inclusion criteria

^bThe corresponding feature was removed in the optimized OPLS-DA model due to a lack of importance

^cStatistics in validation data based on single analysis of each sample

^dAlternatively another less common C11 acylcarnitine isomer

^ePositional isomer not known

^fIsomer not known