FIG. 7.

Knockdown of EGFR-mediated calcium signaling by RNAi. Maximum Fluo-4 fluorescence in cultured U87 human glioma cells is shown after stimulation with 200 ng/ml human EGF. Before measurement of calcium-induced fluorescence the cells were preincubated for 24 h with either vehicle or HIR mAb-targeted PILs carrying clone 967 plasmid DNA. The [Ca2+]_i response in quantified by the number of cells responding to the EGF stimulus. Reproduced with permission⁴¹ (see Table 1 legend).