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. 2015 Dec 15;157(2):913–927. doi: 10.1210/en.2015-1645

Figure 2. Targeted deletion of Bmal1 in specific cellular compartments in the ovary of GCKO and TCKO mice.

Figure 2.

Targeted deletion of Bmal1 in granulosa (GCKO) and theca/stromal (TCKO) cells of the ovary using the CRE:LOX system. Transgenic GCKO (Cyp19-Cre;Bmal1flx/flx) and TCKO (Cyp17-Cre;Bmal1flx/flx) mice were crossed with TOM-GFP mice to produce TCKO-TOM-GFP and GCKO-TOM-GFP transgenic mice (see Supplemental Methods). As shown in A, CRE-driven eGFP expression in GCKO-TOM-GFP is nearly completely limited to the mural and cumulus GC layer with little to any GFP expression detected in the TC/SC compartment or the ovarian surface epithelium (OSE). B, In TCKO-TOM-GFP mice, CRE-driven GFP is more dispersed and spotty in the interstitial cells and limited the TC layer of larger antral follicles with no signal detected in the GC compartment or surface epithelium. Representative images of whole mount (C) pituitary gland and (D) liver from a GCKO mouse. A small number of cells in these tissues from both transgenic strains were positive for GFP (<5%). Scale bars: 0.25 μm shown in A (A and B), 0.05 mm shown in C (C and D), and 0.25 mm shown in E (E and F). Images are representative of 3–4 mice per genotype (LC, GCKO, and TCKO).