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. 2017 Apr 17;12(4):e0176238. doi: 10.1371/journal.pone.0176238

Correction: Efficient and robust differentiation of endothelial cells from human induced pluripotent stem cells via lineage control with VEGF and cyclic AMP

Takeshi Ikuno, Hidetoshi Masumoto, Kohei Yamamizu, Miki Yoshioka, Kenji Minakata, Tadashi Ikeda, Ryuzo Sakata, Jun K Yamashita
PMCID: PMC5393897  PMID: 28414770

The lines labeled “vehicle” and “cAMP+VEGF” are incorrectly switched in Fig 2b and 2c. Please see the corrected Fig 2 here.

Fig 2. Time course of endothelial cell and pre-endothelial cell marker.

Fig 2

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(a) Representative expression time course of VE-cadherin (VECad) and CD31 under stimulation method (VEGF+cAMP) or vehicle without VEGF and cAMP by FACS. (b) Time course of VE-Cadherin-positive cell ratio in two groups. (c) Yield of VE-Cadherin positive endothelial cells per 1cm2 in two groups. (d) Time course of total cell counts in two groups. (e) Representative expression time course of VEGF receptor 2 (VEGFR2) and VE-cadherin in stimulation method (VEGF+cAMP) or vehicle without cAMP and VEGF. Arrows: non-responder cells to VEGF and cAMP stimulation.

Reference

  • 1.Ikuno T, Masumoto H, Yamamizu K, Yoshioka M, Minakata K, Ikeda T, et al. (2017) Efficient and robust differentiation of endothelial cells from human induced pluripotent stem cells via lineage control with VEGF and cyclic AMP. PLoS ONE 12(3): e0173271 doi: 10.1371/journal.pone.0173271 [DOI] [PMC free article] [PubMed] [Google Scholar]

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