Table 1.

Details of the primers used for amplification of different components of CYVMV, CroYVMB, MCS cassette and GFP

Primer name	Primer sequence (5′-3′)	PCR template	Location	Nucleotide co-ordinates	Amplicon size (kb)
BM90F	ATGTCGAAGCGTCCAGCAGAT	CYVMV genome	V2	302-322	0.75
BM82R	TACAGAATCGTAGAAGTAA	Do	V1	1045-1063
BM 638F	CGACGCGTTGTGCGCAATACACTAC	Do	IR	122-141	1.70
BM 639R	GGACTAGTTTAATAAAGATTGAATTTTATTG	Do	C3	1075-1094
BM 640RF	GGTGTACAATTGAATTGGGGACACTCA	Do	IR	2613-2631	0.20
BM 641FR	CCCAAGCTTTGTGCGCAATACACTACTTG	Do	IR	122-141
BM 652F	ACTATCACCCTCAATCACTATAC	Do	C1	1944-1966	3.70
BM 653R	AGAACGGGCAAGACGATG	Do	C1	1926-1943
BM 534F	CARTCATATCCTCCTSYTTGAATTC	CroYVMB	Beta C1	312-336	0.26
BM 535R	CATATATCAGAATGAGACGGGKTTG	Do	AT rich region	745-770
BM 642F	ACGCGTGGACTAGTTTGCATGCCTGCAGGTCC	pRI101	35S Promoter	9009-8991	1.30
BM 643R	TGTACACCCAAGCTTCAGGAAACAGCTATGACCAT	pRI101	NOS terminator	7701-7682
BM 650F	CGGGGTACCGTAGATCTGACTAGTAAAGG	pCAMBIA1302	GFP	4-23	0.77
BM 651F	CGGAATTCGCTAGCTTTGTATAGTTCAT	pCAMBIA1302	GFP	713-733
BM AC3F	CAACCCCTATGTGTTTACAA	CYVMV genome	C3	1284-1303

Bold and italic indicate position of a restriction enzyme site