The first sentence in the Material and Methods section under the subheading "Off-target search" is incorrect. The correct sentence is: For each off-target site of any sgRNA a score is computed that indicates the likelihood of an unstable sgRNA/DNA heteroduplex.
Reference
- 1.Stemmer M, Thumberger T, del Sol Keyer M, Wittbrodt J, Mateo JL (2015) CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool. PLoS ONE 10(4): e0124633 https://doi.org/10.1371/journal.pone.0124633 [DOI] [PMC free article] [PubMed] [Google Scholar]