Table 1.
List of antibodies used for Western blots (WB) and immunofluorescence (IF)
| Antibody against | Dilution for WB | Dilution for IF | Company |
|---|---|---|---|
| Actin | 1:10,000 | Millipore (Temecula CA, USA) | |
| Arginase 1 | 1:1000 | Santa Cruz (Santa Cruz, CA, USA) | |
| Cox-2 | 1:1000 | Cell Signaling Technologies (Danvers, MA, USA) | |
| Glyceraldehyde 3-phosphate dehydrogenase | 1:2000 | Santa Cruz | |
| Glial fibrillary acidic protein | 1:5000 | 1:300 | Cell Signaling Technologies |
| Hemagglutinin antigen HA | 1:1000 | Roche Applied Sciences (Penzberg, Germany) | |
| Human transactive response DNA binding protein 43 (TDP-43; clone 2E2-D3) | 1:1000 | 1:200 | Abnova (Taipei City, Taiwan) |
| Iba-1 | 1:500 | Wako Chemicals (Richmond, VA, USA) | |
| IkBa | 1:1000 | Santa Cruz | |
| Inducible nitric oxide synthase | 1:1000 | BD Biosciences (San Jose, CA, USA) | |
| NeuN | 1:500 | Cell Signaling Technologies | |
| Neurofilament-H | 1:100 | Millipore | |
| Nuclear factor kappa B (NF-κB) | 1:1000 | Santa Cruz | |
| P84 | 1:2500 | Abcam (Cambridge, UK) | |
| Pan-TDP-43 | 1:5000 | Proteintech (Chicago, IL, USA) | |
| Peripherin | 1:5000 | Millipore | |
| Phospho-NF-κB (Ser 536) | 1:250 | Cell Signaling Technologies, | |
| Synaptic vesicle protein-2 SV-2 | 1:25 | Developmental Studies Hybridoma Bank (Mt. Prospect, IA, USA) | |
| Tumor necrosis factor-α | 1:400 | Abcam | |
| Ym-1 | 1:500 | Stem Cell Technologies (Vancouver, Canada) |