Figure 2.

Food intake, body weight, body composition, daily rhythm of respiratory quotient, plasma triglycerides and FFAs, and glycogen content in liver and skeletal muscle after 7 days of daily treatment with an SGLT2-I. ZDF and ZCL rats were treated from 9 weeks of age with vehicle (ZDF-V7 and ZCL-V7, respectively) or an SGLT2-I (ZDF-CA7) once daily for 7 days. A: Oxygen consumption and CO₂ production were monitored at 1-min intervals every 15 min for 24 h (from 6:00 a.m. on day 7 to 8:00 a.m. on day 8). B: Food intake during the light phase (6:00 a.m. to 6:00 p.m.) and the dark phase (6:00 p.m. to 6:00 a.m.) on day 7 of the treatments. C: Body weight before initiation of the treatment (at 9:00 a.m. on day 1) and after 7 days of the treatment (at 9:00 a.m. on day 7). D and E: Body composition measured between 9:00 and 11:00 a.m. on day 7 of treatment. F: Plasma triglycerides and FFAs. G: Triglyceride content in the liver and skeletal muscle. G-P, gastrocnemius-plantaris; S, soleus. H: Glycogen content in the liver and skeletal muscle (vastus lateralis). Blood and these tissues were collected between 12:00 and 1:00 p.m. on day 8. Values are the mean ± SEM of data from eight animals in each group. *Significant difference from the corresponding values of the ZCL-V7 group (P < 0.05); †significant difference from the corresponding values of the ZDF-V7 group (P < 0.05).