Table 3.

Summary of the coupling energy (ΔΔG_int) measured for each pair of residues. ΔΔG_int values are in kcal/mol and were calculated according to the description given in the Materials and methods section. Average and standard errors (SE) are shown. The gray cells indicate mutations of YAP and TEAD in the same binding pocket. n. m. (not measured) indicates that ΔΔG_int could not be determined experimentally because the K_d values for the mutant YAP:mutant TEAD interaction in the corresponding double mutant cycles were above our assay limit (>200 µM).

DOI: http://dx.doi.org/10.7554/eLife.25068.007

			hYAP50-171
			α-helix			Ω-loop
			Leu65	Leu68	Phe69	Met86	Arg89	Leu91	Ser94	Phe95	Phe96
hTEAD217-434	α-helix	Phe337	−0.34 ±0.04	−0.65 ±0.04	−0.96 ±0.04	0.17 ±0.04	−0.44 ±0.03	n. m.	−0.09 ±0.05	n. m.	0.02 ±0.06
		Lys376	−0.65 ±0.04	−0.41 ±0.05	−1.28 ±0.06	0.13 ±0.04	−0.62 ±0.03	−0.41 ±0.06	−0.22 ±0.06	−0.30 ±0.04	−0.14 ±0.05
		Leu380	−0.36 ±0.05	−0.29 ±0.04	−1.01 ±0.06	0.07 ±0.03	−0.29 ±0.03	−0.26 ±0.05	−0.08 ±0.05	−0.18 ±0.03	−0.02 ±0.06
		Val389	−0.52 ±0.06	−0.29 ±0.05	−1.11 ±0.05	0.16 ±0.03	n. m.	n. m.	0.03 ±0.05	n. m.	−0.06 ±0.04
	Ω-loop	Glu263	−0.05 ±0.05	−0.09 ±0.03	−0.10 ±0.06	0.07 ±0.05	−0.59 ±0.06	−0.62 ±0.05	−0.96 ±0.05	−0.51 ±0.05	−0.04 ±0.07
		Val265	0.26 ±0.03	0.15 ±0.03	0.13 ±0.05	0.09 ±0.03	−0.58 ±0.02	−0.28 ±0.06	−0.06 ±0.06	−0.61 ±0.04	−0.22 ±0.06
		Asp272	n. m.	−0.02 ±0.04	n. m.	−1.85 ±0.04	−3.49 ±0.05	−2.74 ±0.06	−1.65 ±0.05	−2.50 ±0.07	−1.67 ±0.06