Figure 6. ANXA13 upregulates active MMP-9 through AKT phosphorylation.

(A) Zymographic assay showing that the level of active MMP-9 was upregulated by ANXA13 overexpression but downregulated by ANXA13-siRNA. (B) Western blot showing that ANXA13-mediated MMP-9 expression was abolished by AKT inhibitor LY29004, which inhibited AKT kinase function. Values are mean ± SEM; n = 3; *P < 0.05, **P < 0.01 by unpaired Student's t test.