Abstract
Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 microM AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.
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- Bytebier B., Deboeck F., De Greve H., Montagu M. V., Hernalsteens J. P. T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5345–5349. doi: 10.1073/pnas.84.15.5345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chilton M. D., Saiki R. K., Yadav N., Gordon M. P., Quetier F. T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060–4064. doi: 10.1073/pnas.77.7.4060. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Corcuera L. J., Woodward M. D., Helgeson J. P., Kelman A., Upper C. D. 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, an Inhibitor from Zea mays with Differential Activity against Soft Rotting Erwinia Species. Plant Physiol. 1978 May;61(5):791–795. doi: 10.1104/pp.61.5.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donson J., Gunn H. V., Woolston C. J., Pinner M. S., Boulton M. I., Mullineaux P. M., Davies J. W. Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA. Virology. 1988 Jan;162(1):248–250. doi: 10.1016/0042-6822(88)90416-3. [DOI] [PubMed] [Google Scholar]
- Gasser C. S., Fraley R. T. Genetically engineering plants for crop improvement. Science. 1989 Jun 16;244(4910):1293–1299. doi: 10.1126/science.244.4910.1293. [DOI] [PubMed] [Google Scholar]
- Huang Y., Morel P., Powell B., Kado C. I. VirA, a coregulator of Ti-specified virulence genes, is phosphorylated in vitro. J Bacteriol. 1990 Feb;172(2):1142–1144. doi: 10.1128/jb.172.2.1142-1144.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jin S., Roitsch T., Ankenbauer R. G., Gordon M. P., Nester E. W. The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation. J Bacteriol. 1990 Feb;172(2):525–530. doi: 10.1128/jb.172.2.525-530.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sciaky D., Montoya A. L., Chilton M. D. Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978 Feb;1(2):238–253. doi: 10.1016/0147-619x(78)90042-2. [DOI] [PubMed] [Google Scholar]
- Stachel S. E., An G., Flores C., Nester E. W. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985 Apr;4(4):891–898. doi: 10.1002/j.1460-2075.1985.tb03715.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stachel S. E., Nester E. W. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1986 Jul;5(7):1445–1454. doi: 10.1002/j.1460-2075.1986.tb04381.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stachel S. E., Nester E. W., Zambryski P. C. A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proc Natl Acad Sci U S A. 1986 Jan;83(2):379–383. doi: 10.1073/pnas.83.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tipton C. L., Klun J. A., Husted R. R., Pierson M. D. Cyclic hydroxamic acids and related compounds from maize. Isolation and characterization. Biochemistry. 1967 Sep;6(9):2866–2870. doi: 10.1021/bi00861a030. [DOI] [PubMed] [Google Scholar]
- Winans S. C., Kerstetter R. A., Nester E. W. Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens. J Bacteriol. 1988 Sep;170(9):4047–4054. doi: 10.1128/jb.170.9.4047-4054.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodward M. D., Corcuera L. J., Helgeson J. P., Kelman A., Upper C. D. Factors That Influence the Activity of 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one on Erwinia Species in Growth Assays. Plant Physiol. 1978 May;61(5):803–805. doi: 10.1104/pp.61.5.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodward M. D., Corcuera L. J., Helgeson J. P., Kelman A., Upper C. D. Quantitation of 1,4-Benzoxazin-3-ones in Maize by Gas-Liquid Chromatography. Plant Physiol. 1979 Jan;63(1):14–19. doi: 10.1104/pp.63.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodward M. D., Corcuera L. J., Helgeson J. P., Upper C. D. Decomposition of 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in Aqueous Solutions. Plant Physiol. 1978 May;61(5):796–802. doi: 10.1104/pp.61.5.796. [DOI] [PMC free article] [PubMed] [Google Scholar]