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. 1990 Jun;87(11):4038–4042. doi: 10.1073/pnas.87.11.4038

Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability.

J E Mullet 1, P G Klein 1, R R Klein 1
PMCID: PMC54042  PMID: 2349216

Abstract

Chlorophyll apoprotein accumulation in higher plant chloroplasts is controlled by light-dependent chlorophyll formation. Dark-grown plants lack chlorophyll and chlorophyll apoproteins. However, the plastid genes encoding the chlorophyll apoproteins are transcribed; chlorophyll apoprotein mRNA accumulates and associates with polysomes in plastids of dark-grown plants. Pulse-labeling assays revealed a population of short-lived proteins in plastids of dark-grown plants. One of these transiently labeled proteins was CP43, a chlorophyll apoprotein associated with photosystem II. Pulse-chase assays showed that newly synthesized CP43 was rapidly degraded in plastids of dark-grown plants, which lack chlorophyll. In contrast, CP43 synthesized in plastids from illuminated plants was stable. The synthesis of D1, a chlorophyll apoprotein of the photosystem II reaction center, was also analyzed in plastids of dark-grown and illuminated plants. Radiolabel accumulation into full-length D1 was only detected in plastids of illuminated plants. However, D1 translation intermediates of 15-25 kDa were detected in both plastid populations. Pulse-chase assays showed that the 15- to 25-kDa D1 translation products were precursors of mature D1 in plastids of illuminated plants. In contrast, in plastids of dark-grown plants, the 15- to 25-kDa translation intermediates were converted into a 23-kDa polypeptide previously suggested to be a proteolytic product of D1. These results indicate that chlorophyll produced in illuminated plants stabilizes D1 nascent polypeptides, which allows accumulation of mature D1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apel K. Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare). Eur J Biochem. 1979 Jun;97(1):183–188. doi: 10.1111/j.1432-1033.1979.tb13101.x. [DOI] [PubMed] [Google Scholar]
  2. Bennett J. Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness. Eur J Biochem. 1981 Aug;118(1):61–70. doi: 10.1111/j.1432-1033.1981.tb05486.x. [DOI] [PubMed] [Google Scholar]
  3. Berends T., Gamble P. E., Mullet J. E. Characterization of the barley chloroplast transcription units containing psaA-psaB and psbD-psbC. Nucleic Acids Res. 1987 Jul 10;15(13):5217–5240. doi: 10.1093/nar/15.13.5217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fish L. E., Kück U., Bogorad L. Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I. J Biol Chem. 1985 Feb 10;260(3):1413–1421. [PubMed] [Google Scholar]
  5. Gamble P. E., Mullet J. E. Translation and stability of proteins encoded by the plastid psbA and psbB genes are regulated by a nuclear gene during light-induced chloroplast development in barley. J Biol Chem. 1989 May 5;264(13):7236–7243. [PubMed] [Google Scholar]
  6. Greenberg B. M., Gaba V., Mattoo A. K., Edelman M. Identification of a primary in vivo degradation product of the rapidly-turning-over 32 kd protein of photosystem II. EMBO J. 1987 Oct;6(10):2865–2869. doi: 10.1002/j.1460-2075.1987.tb02588.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ikeuchi M., Plumley F. G., Inoue Y., Schmidt G. W. Phosphorylation of Photosystem II Components, CP43 Apoprotein, D1, D2, and 10 to 11 Kilodalton Protein in Chloroplast Thylakoids of Higher Plants. Plant Physiol. 1987 Nov;85(3):638–642. doi: 10.1104/pp.85.3.638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein R. R., Gamble P. E., Mullet J. E. Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant Physiol. 1988 Dec;88(4):1246–1256. doi: 10.1104/pp.88.4.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein R. R., Mason H. S., Mullet J. E. Light-regulated translation of chloroplast proteins. I. Transcripts of psaA-psaB, psbA, and rbcL are associated with polysomes in dark-grown and illuminated barley seedlings. J Cell Biol. 1988 Feb;106(2):289–301. doi: 10.1083/jcb.106.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein R. R., Mullet J. E. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings. J Biol Chem. 1987 Mar 25;262(9):4341–4348. [PubMed] [Google Scholar]
  11. Klein R. R., Mullet J. E. Regulation of chloroplast-encoded chlorophyll-binding protein translation during higher plant chloroplast biogenesis. J Biol Chem. 1986 Aug 25;261(24):11138–11145. [PubMed] [Google Scholar]
  12. Kreuz K., Dehesh K., Apel K. The light-dependent accumulation of the P700 chlorophyll a protein of the photosystem I reaction center in barley. Evidence for translational control. Eur J Biochem. 1986 Sep 15;159(3):459–467. doi: 10.1111/j.1432-1033.1986.tb09908.x. [DOI] [PubMed] [Google Scholar]
  13. Marder J. B., Goloubinoff P., Edelman M. Molecular architecture of the rapidly metabolized 32-kilodalton protein of photosystem II. Indications for COOH-terminal processing of a chloroplast membrane polypeptide. J Biol Chem. 1984 Mar 25;259(6):3900–3908. [PubMed] [Google Scholar]
  14. Mattoo A. K., Marder J. B., Edelman M. Dynamics of the photosystem II reaction center. Cell. 1989 Jan 27;56(2):241–246. doi: 10.1016/0092-8674(89)90897-0. [DOI] [PubMed] [Google Scholar]
  15. Merchant S., Bogorad L. Rapid degradation of apoplastocyanin in Cu(II)-deficient cells of Chlamydomonas reinhardtii. J Biol Chem. 1986 Dec 5;261(34):15850–15853. [PubMed] [Google Scholar]
  16. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mullet J. E., Grossman A. R., Chua N. H. Synthesis and assembly of the polypeptide subunits of photosystem I. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 2):979–984. doi: 10.1101/sqb.1982.046.01.091. [DOI] [PubMed] [Google Scholar]
  18. Mullet J. E., Klein R. R., Grossman A. R. Optimization of protein synthesis in isolated higher plant chloroplasts. Identification of paused translation intermediates. Eur J Biochem. 1986 Mar 3;155(2):331–338. doi: 10.1111/j.1432-1033.1986.tb09495.x. [DOI] [PubMed] [Google Scholar]
  19. Mullet J. E., Klein R. R. Transcription and RNA stability are important determinants of higher plant chloroplast RNA levels. EMBO J. 1987 Jun;6(6):1571–1579. doi: 10.1002/j.1460-2075.1987.tb02402.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oelze J., Schroeder J., Drews G. Bacteriochlorophyll, fatty-acid, and protein synthesis in relation to thylakoid formation in mutant strains of Rhodospirillum rubrum. J Bacteriol. 1970 Mar;101(3):669–674. doi: 10.1128/jb.101.3.669-674.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Renger G., Wolff C. Further evidence for dissipative energy migration via triplet states in photosynthesis. The protective mechanism of carotenoids in Rhodopseudomonas spheroides chromatophores. Biochim Biophys Acta. 1977 Apr 11;460(1):47–57. doi: 10.1016/0005-2728(77)90150-5. [DOI] [PubMed] [Google Scholar]
  22. Sayre R. T., Andersson B., Bogorad L. The topology of a membrane protein: the orientation of the 32 kd Qb-binding chloroplast thylakoid membrane protein. Cell. 1986 Nov 21;47(4):601–608. doi: 10.1016/0092-8674(86)90624-0. [DOI] [PubMed] [Google Scholar]
  23. Sutton A., Sieburth L. E., Bennett J. Light-dependent accumulation and localization of photosystem II proteins in maize. Eur J Biochem. 1987 May 4;164(3):571–578. doi: 10.1111/j.1432-1033.1987.tb11165.x. [DOI] [PubMed] [Google Scholar]
  24. Takemoto J., Lascelles J. Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas spheroides. Proc Natl Acad Sci U S A. 1973 Mar;70(3):799–803. doi: 10.1073/pnas.70.3.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Westhoff P., Alt J., Herrmann R. G. Localization of the genes for the two chlorophyll a-conjugated polypeptides (mol. wt. 51 and 44 kd) of the photosystem II reaction center on the spinach plastid chromosome. EMBO J. 1983;2(12):2229–2237. doi: 10.1002/j.1460-2075.1983.tb01728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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