Figure 4. Bilateral inputs to the VPM in Robo3R3-5-cKO mice.
Whole brain iDISCO+ scans from adult mice immunolabeled for c-Fos. The whiskers were shaved on the left side, and b, d rows were spared on the right side. (A) Presentation of the projection plane used in the following panels: a 45° oblique (from both coronal and sagittal) 50 µm projection plane was positioned to intersect with the VPM annotation (in red). (B) Details of the c-Fos pattern in the VPM of Robo3R3-5-Het and Robo3R3-5-cKO mice on each side. In controls, two bands of c-Fos+ cells were seen on the side contralateral to the spared whiskers, revealing the b and d row barreloids. In Robo3R3-5-cKO mice, a dual band pattern in the VPM was seen on both sides of the brain. On the ipsilateral side, the bands were visible on the same intersecting plane as in the controls. On the contralateral side, the bands of activity were visible on a more medial plane, at the edge of the VPM annotation. (C) Representation of the VPM organization in the Robo3R3-5-cKO mice. Scale bars are 500 µm.