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(A) SHAPE reactivity profile of the AUG1AUG2 (black) and AUG1GCG2 (blue) domain I as a function of nucleotide position. Nucleotides that changed their reactivity in domain IGCG2 are indicated. (B) Native gel electrophoresis of the [32P]-labeled wild-type and mutated domain I of Ty1i RNA at increasing concentrations of MgCl2. C: control reaction without MgCl2. WT: wild type.
