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. 1990 Jun;87(11):4256–4260. doi: 10.1073/pnas.87.11.4256

Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

H M Albertsen 1, H Abderrahim 1, H M Cann 1, J Dausset 1, D Le Paslier 1, D Cohen 1
PMCID: PMC54087  PMID: 2190217

Abstract

Prior to constructing a library of yeast artificial chromosomes (YACs) containing very large human DNA fragments, we performed a series of preliminary experiments aimed at developing a suitable protocol. We found an inverse relationship between YAC insert size and transformation efficiency. Evidence of occasional rearrangement within YAC inserts was found resulting in clonally stable internal deletions or clonally unstable size variations. A protocol was developed for preparative electrophoretic enrichment of high molecular mass human DNA fragments from partial restriction digests and ligation with the YAC vector in agarose. A YAC library has been constructed from large fragments of DNA from an Epstein-Barr virus-transformed human lymphoblastoid cell line. The library presently contains 50,000 clones, 95% of which are greater than 250 kilobase pairs in size. The mean YAC size of the library, calculated from 132 randomly isolated clones, is 430 kilobase pairs. The library thus contains the equivalent of approximately seven haploid human genomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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