Figure 6. Knockdown of PLOD2 inhibits glioma cell invasion in vivo.

(A) Nude mice bearing subcutaneous xenografts of injected U251-shPLOD2 or U251-shNC cells (U251-shPLOD2, n = 5; U251-shNC, n = 5). Subcutaneous xenograft tumor volume (mm³) was measured on day 43 with a caliper. (B) Concentration of hydroxyproline (μg/mL) in U251-shNC or U251-shPLOD2 subcutaneous xenografts as determined by ELISA. (C) Stiffness of U251-shNC or U251-shPLOD2 subcutaneous xenografts measured with the compression test. Measurements were made on 3 randomly chosen sites in each tumor. ns, not significant, Student's t test. (D) PLOD2 immunohistochemical staining of tissue sections from U251-shNC or U251-shPLOD2 orthotopic xenografts (Scale bars, 100 μm). (E) Hematoxylin and eosin staining of sections at tumor margins in intracranial U251-shNC or U251-shPLOD2 xenografts (Scale bars, 100 μm). (F) Picrosirius red staining of tumor sections from U251-shNC or U251-shPLOD2 intracranial xenografts captured under bright and polarized light (Scale bars, 100 μm).