Figure 5. DNA damage in response to evofosfamide and irradiation.
(A) Residual γH2AX foci were analyzed in A549 cells treated for 4 hours with evofosfamide and irradiation with 2 Gy. Cells were analyzed 20 hours after irradiation at a magnification of 40x. (B) Induction of senescence (β-galactosidase staining) in response to the combined treatment in A549 cells. Cells were analyzed 72 hours after treatment at a magnification of 10x. At least 50 cells/condition were analyzed. Representative pictures are shown. Error bars represent SEM.