Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Jun;87(12):4417–4420. doi: 10.1073/pnas.87.12.4417

Introduction of the beta isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells.

E Melloni 1, S Pontremoli 1, B Sparatore 1, M Patrone 1, F Grossi 1, P A Marks 1, R A Rifkind 1
PMCID: PMC54125  PMID: 2352925

Abstract

Induction of differentiation in murine erythroleukemia cells (MELCs) involves a protein kinase C (PKC)-mediated step. Vincristine-resistant cells respond more rapidly to hybrid polar/apolar inducers than the parental cells. These vincristine-resistant MELCs contain elevated levels of the beta isozyme of PKC (PKC-beta). Exogenous homologous murine PKC-beta, incorporated into permeabilized MELCs, accelerates induced differentiation. Neither rat PKC-beta, nor mouse PKC-alpha, nor rat PKC-alpha, incorporated into permeabilized MELCs, is effective in altering the kinetics of induced differentiation. This provides direct evidence for a rate-limiting role for this PKC isozyme during N,N'-hexamethylenebisacetamide-mediated induced differentiation of a transformed cell.

Full text

PDF
4417

Images in this article

4418Image
on p.4418

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balazovich K. J., Portnow D., Boxer L. A., Prochownik E. V. Changes in protein kinase C activity are associated with the differentiation of Friend erythroleukemia cells. Biochim Biophys Acta. 1987 Feb 18;927(2):247–255. doi: 10.1016/0167-4889(87)90141-8. [DOI] [PubMed] [Google Scholar]
  2. Bernstein A., Hunt D. M., Crichley V., Mak T. W. Induction by ouabain of hemoglobin synthesis in cultured Friend erythroleukemic cells. Cell. 1976 Nov;9(3):375–381. doi: 10.1016/0092-8674(76)90082-9. [DOI] [PubMed] [Google Scholar]
  3. Bridges K., Levenson R., Housman D., Cantley L. Calcium regulates the commitment of murine erythroleukemia cells to terminal erythroid differentiation. J Cell Biol. 1981 Aug;90(2):542–544. doi: 10.1083/jcb.90.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen Z., Banks J., Rifkind R. A., Marks P. A. Inducer-mediated commitment of murine erythroleukemia cells to differentiation: a multistep process. Proc Natl Acad Sci U S A. 1982 Jan;79(2):471–475. doi: 10.1073/pnas.79.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fibach E., Reuben R. C., Rifkind R. A., Marks P. A. Effect of hexamethylene bisacetamide on the commitment to differentiation of murine erythroleukemia cells. Cancer Res. 1977 Feb;37(2):440–444. [PubMed] [Google Scholar]
  6. Gazitt Y., Deitch A. D., Marks P. A., Rifkind R. A. Cell volume changes in relation to the cell cycle of differentiating erythroleukemic cells. Exp Cell Res. 1978 Dec;117(2):413–420. doi: 10.1016/0014-4827(78)90154-4. [DOI] [PubMed] [Google Scholar]
  7. Gazitt Y., Reuben R. C., Deitch A. D., Marks P. A., Rifkind R. A. Changes in cyclic adenosine 3':5'-monophosphate levels during induction of differentiation in murine erythroleukemia cells. Cancer Res. 1978 Nov;38(11 Pt 1):3779–3783. [PubMed] [Google Scholar]
  8. Gusella J., Geller R., Clarke B., Weeks V., Housman D. Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. Cell. 1976 Oct;9(2):221–229. doi: 10.1016/0092-8674(76)90113-6. [DOI] [PubMed] [Google Scholar]
  9. Gusella J., Geller R., Clarke B., Weeks V., Housman D. Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. Cell. 1976 Oct;9(2):221–229. doi: 10.1016/0092-8674(76)90113-6. [DOI] [PubMed] [Google Scholar]
  10. Kikkawa U., Go M., Koumoto J., Nishizuka Y. Rapid purification of protein kinase C by high performance liquid chromatography. Biochem Biophys Res Commun. 1986 Mar 13;135(2):636–643. doi: 10.1016/0006-291x(86)90040-9. [DOI] [PubMed] [Google Scholar]
  11. Lachman H. M., Skoultchi A. I. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells. Nature. 1984 Aug 16;310(5978):592–594. doi: 10.1038/310592a0. [DOI] [PubMed] [Google Scholar]
  12. Mager D., Bernstein A. The program of Friend cell erythroid differentiation: early changes in Na+/K+ ATPase function. J Supramol Struct. 1978;8(4):431–438. doi: 10.1002/jss.400080405. [DOI] [PubMed] [Google Scholar]
  13. Makowske M., Ballester R., Cayre Y., Rosen O. M. Immunochemical evidence that three protein kinase C isozymes increase in abundance during HL-60 differentiation induced by dimethyl sulfoxide and retinoic acid. J Biol Chem. 1988 Mar 5;263(7):3402–3410. [PubMed] [Google Scholar]
  14. Marks P. A., Breslow R., Rifkind R. A., Ngo L., Singh R. Polar/apolar chemical inducers of differentiation of transformed cells: strategies to improve therapeutic potential. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6358–6362. doi: 10.1073/pnas.86.16.6358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marks P. A., Sheffery M., Rifkind R. A. Induction of transformed cells to terminal differentiation and the modulation of gene expression. Cancer Res. 1987 Feb 1;47(3):659–666. [PubMed] [Google Scholar]
  16. Melloni E., Pontremoli S., Damiani G., Viotti P., Weich N., Rifkind R. A., Marks P. A. Vincristine-resistant erythroleukemia cell line has marked increased sensitivity to hexamethylenebisacetamide-induced differentiation. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3835–3839. doi: 10.1073/pnas.85.11.3835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Melloni E., Pontremoli S., Michetti M., Sacco O., Cakiroglu A. G., Jackson J. F., Rifkind R. A., Marks P. A. Protein kinase C activity and hexamethylenebisacetamide-induced erythroleukemia cell differentiation. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5282–5286. doi: 10.1073/pnas.84.15.5282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Melloni E., Pontremoli S., Viotti P. L., Patrone M., Marks P. A., Rifkind R. A. Differential expression of protein kinase C isozymes and erythroleukemia cell differentiation. J Biol Chem. 1989 Nov 5;264(31):18414–18418. [PubMed] [Google Scholar]
  19. Nishizuka Y. Studies and prospectives of the protein kinase c family for cellular regulation. Cancer. 1989 May 15;63(10):1892–1903. doi: 10.1002/1097-0142(19890515)63:10<1892::aid-cncr2820631005>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  20. Nomura S., Kamiya T., Oishi M. A procedure to introduce protein molecules into living mammalian cells. Exp Cell Res. 1986 Apr;163(2):434–444. doi: 10.1016/0014-4827(86)90074-1. [DOI] [PubMed] [Google Scholar]
  21. Ramsay R. G., Ikeda K., Rifkind R. A., Marks P. A. Changes in gene expression associated with induced differentiation of erythroleukemia: protooncogenes, globin genes, and cell division. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6849–6853. doi: 10.1073/pnas.83.18.6849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reuben R. C., Wife R. L., Breslow R., Rifkind R. A., Marks P. A. A new group of potent inducers of differentiation in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):862–866. doi: 10.1073/pnas.73.3.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sekiguchi K., Tsukuda M., Ase K., Kikkawa U., Nishizuka Y. Mode of activation and kinetic properties of three distinct forms of protein kinase C from rat brain. J Biochem. 1988 May;103(5):759–765. doi: 10.1093/oxfordjournals.jbchem.a122343. [DOI] [PubMed] [Google Scholar]
  24. Todokoro K., Ikawa Y. Sequential expression of proto-oncogenes during a mouse erythroleukemia cell differentiation. Biochem Biophys Res Commun. 1986 Mar 28;135(3):1112–1118. doi: 10.1016/0006-291x(86)91043-0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES