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. 1975 Jul;56(1):121–125. doi: 10.1104/pp.56.1.121

Phosphate-dependent Substrate Transport into Mitochondria

Oxidative Studies 1

Joseph T Wiskich a
PMCID: PMC541310  PMID: 16659240

Abstract

The oxidation of malate and citrate by isolated plant mitochondria can be stimulated by the addition of inorganic phosphate. This stimulation (a) is not inhibited by oligomycin or uncouplers; (b) can not be duplicated by addition of adenine nucleotide; (c) is inhibited by 2-n-butylmalonate; and (d) is not evident in detergent-treated mitochondria. Phosphate was required to elicit uncoupler-stimulated respiration. It is concluded that these effects of phosphate are attributable to a stimulated rate of substrate penetration into the mitochondria, and do not involve the oxidative phosphorylation process.

Disrupting the mitochondria with detergent removed the requirement for phosphate and showed that butylmalonate did not inhibit the respiratory enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANCE B., WILLIAMS G. R. A method for the localization of sites for oxidative phosphorylation. Nature. 1955 Aug 6;176(4475):250–254. doi: 10.1038/176250a0. [DOI] [PubMed] [Google Scholar]
  2. Chappell J. B. Systems used for the transport of substrates into mitochondria. Br Med Bull. 1968 May;24(2):150–157. doi: 10.1093/oxfordjournals.bmb.a070618. [DOI] [PubMed] [Google Scholar]
  3. Day D. A., Wiskich J. T. The oxidation of malate and exogenous reduced nicotinamide adenine dinucleotide by isolated plant mitochondria. Plant Physiol. 1974 Jan;53(1):104–109. doi: 10.1104/pp.53.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanson J. B., Bertagnolli B. L., Shepherd W. D. Phosphate-induced Stimulation of Acceptorless Respiration in Corn Mitochondria. Plant Physiol. 1972 Sep;50(3):347–354. doi: 10.1104/pp.50.3.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Laties G. G. The potentiating effect of adenosine diphosphate in the uncoupling of oxidative phosphorylation in potato mitochondria. Biochemistry. 1973 Aug 14;12(17):3350–3355. doi: 10.1021/bi00741a032. [DOI] [PubMed] [Google Scholar]
  7. Millard D. L., Wiskich J. T., Robertson R. N. ION UPTAKE BY PLANT MITOCHONDRIA. Proc Natl Acad Sci U S A. 1964 Oct;52(4):996–1004. doi: 10.1073/pnas.52.4.996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Millard D. L., Wiskich J. T., Robertson R. N. Ion Uptake and Phosphorylation in Mitochondria: Effect of Monovalent Ions. Plant Physiol. 1965 Nov;40(6):1129–1135. doi: 10.1104/pp.40.6.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Phillips M. L., Williams G. R. Anion transporters in plant mitochondria. Plant Physiol. 1973 Apr;51(4):667–670. doi: 10.1104/pp.51.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Phillips M. L., Williams G. R. Effects of 2-Butylmalonate, 2-Phenylsuccinate, Benzylmalonate, and p-Iodobenzylmalonate on the Oxidation of Substrates by Mung Bean Mitochondria. Plant Physiol. 1973 Feb;51(2):225–228. doi: 10.1104/pp.51.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robinson B. H., Chappell J. B. The inhibition of malate, tricarboxylate and oxoglutarate entry into mitochondria by 2-n-butylmalonate. Biochem Biophys Res Commun. 1967 Jul 21;28(2):249–255. doi: 10.1016/0006-291x(67)90437-8. [DOI] [PubMed] [Google Scholar]
  12. Sauer L. A., Park R. A stimulation by phosphate of malate transport and oxidation in rat adrenal mitochondria. Biochemistry. 1973 Feb;12(4):643–649. doi: 10.1021/bi00728a012. [DOI] [PubMed] [Google Scholar]

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