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. 1974 Feb;53(2):167–170. doi: 10.1104/pp.53.2.167

Enzymes Related to Lactate Metabolism in Green Algae and Lower Land Plants 1

Peter J Gruber a,2, Sue Ellen Frederick a,2, N E Tolbert a
PMCID: PMC541358  PMID: 16658670

Abstract

Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD+-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD+-d-lactate dehydrogenase being soluble. The reduction of pyruvate by NADH proceeded more rapidly than the reverse reaction, and the apparent Michaelis constants for pyruvate and NADH were lower than for d-lactate and NAD+. These data indicated that under physiological conditions, the NAD+-linked d-lactate dehydrogenase probably functions to produce d-lactate from pyruvate.

Lactate dehydrogenase activity dependent on NAD+ was found in a number of other green algae and in the green tissues of a few lower land plants. When present in species which contain glycolate oxidase rather than glycolate dehydrogenase, the enzyme was specific for l-lactate rather than d-lactate. A cyclic system revolving around the production and utilization of d-lactate in some species and l-lactate in certain others is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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