Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1974 Apr;53(4):543–554. doi: 10.1104/pp.53.4.543

The Chloroplast and Cytoplasmic Ribosomes of Euglena

II. Characterization of Ribosomal Proteins 1

Georges Freyssinet a,2, Jerome A Schiff a
PMCID: PMC541395  PMID: 16658741

Abstract

Cytoplasmic and chloroplast ribosomal proteins were isolated from Euglena gracilis and analyzed on polyacrylamide gels. Cytoplasmic ribosomes appear to contain 75 to 100 proteins ranging in molecular weight from 10,200 to 104,000, while chloroplast ribosomes appear to contain 35 to 42 proteins with molecular weights ranging from 9,700 to 57,900. This indicates that the cytoplasmic ribosomes are similar in composition to other eucaryotic ribosomes, while chloroplast ribosomes have a protein composition similar to the 70S procaryotic ribosome. The kinetics of light-induced labeling of cytoplasmic ribosomal proteins during chloroplast development has been determined, and the results are compared with the kinetics of ribosomal RNA synthesis.

Full text

PDF
543

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avadhani N. G., Buetow D. E. Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis. Biochem J. 1972 Jun;128(2):353–365. doi: 10.1042/bj1280353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bickle T. A., Traut R. R. Differences in size and number of 80 S and 70 S ribosomal proteins by dodecyl sulfate gel electrophoresis. J Biol Chem. 1971 Nov 25;246(22):6828–6834. [PubMed] [Google Scholar]
  3. Brown R. D., Haselkorn R. Synthesis and maturation of cytoplasmic ribosomal RNA in Euglena gracilis. J Mol Biol. 1971 Aug 14;59(3):491–503. doi: 10.1016/0022-2836(71)90312-3. [DOI] [PubMed] [Google Scholar]
  4. Delihas N., Jupp A., Lyman H. Properties of Euglena gracilis cytoplasmic ribosomes in salt. Biochim Biophys Acta. 1972 Mar 24;262(3):344–351. doi: 10.1016/0005-2787(72)90272-9. [DOI] [PubMed] [Google Scholar]
  5. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gnanam A., Kahn J. S. Biochemical studies on the induction of chloroplast development in Euglena gracilis. 3. Ribosome metabolism associated with chloroplast development. Biochim Biophys Acta. 1967 Jul 18;142(2):493–499. doi: 10.1016/0005-2787(67)90630-2. [DOI] [PubMed] [Google Scholar]
  7. Heizmann P., Trabuchet G., Verdier G., Freyssinet G., Nigon V. Influence de l'éclairement sur l'évolution des polysomes dans des cultures d'Euglena gracilis étiolées. Biochim Biophys Acta. 1972 Aug 16;277(1):149–160. [PubMed] [Google Scholar]
  8. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  9. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes. Biochimie. 1972;54(2):167–175. doi: 10.1016/s0300-9084(72)80101-9. [DOI] [PubMed] [Google Scholar]
  10. King H. W., Gould H. J., Shearman J. J. Molecular weight distribution of proteins in rabbit reticulocyte ribosomal subunits. J Mol Biol. 1971 Oct 14;61(1):143–156. doi: 10.1016/0022-2836(71)90212-9. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lefkovits I., Di Girolamo M. Reutilization of ribosomal proteins in vivo for the formation of new ribosomal particles in Escherichia coli B. Biochim Biophys Acta. 1969 Feb 18;174(2):566–573. doi: 10.1016/0005-2787(69)90286-x. [DOI] [PubMed] [Google Scholar]
  13. Low R. B., Wool I. G. Mammalian ribosomal protein: analysis by electrophoresis on polyacrylamide gel. Science. 1967 Jan 20;155(3760):330–332. doi: 10.1126/science.155.3760.330. [DOI] [PubMed] [Google Scholar]
  14. Lyttleton J. W. Protein constituents of plant ribosomes. Biochim Biophys Acta. 1968 Jan 22;154(1):145–149. doi: 10.1016/0005-2795(68)90266-3. [DOI] [PubMed] [Google Scholar]
  15. Martini O. H., Gould H. J. Enumeration of rabbit reticulocyte ribosomal proteins. J Mol Biol. 1971 Dec 14;62(2):403–405. doi: 10.1016/0022-2836(71)90435-9. [DOI] [PubMed] [Google Scholar]
  16. Schwartzbach S. D., Freyssinet G., Schiff J. A. The chloroplast and cytoplasmic ribosomes of euglena: I. Stability of chloroplast ribosomes prepared by an improved procedure. Plant Physiol. 1974 Apr;53(4):533–542. doi: 10.1104/pp.53.4.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sherton C. C., Wool I. G. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem. 1972 Jul 25;247(14):4460–4467. [PubMed] [Google Scholar]
  18. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  19. Welfle H., Stahl J., Bielka H. Studies on proteins of animal ribosomes. 8. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins of rat liver. Biochim Biophys Acta. 1971 Sep 28;243(3):416–419. doi: 10.1016/0005-2795(71)90009-2. [DOI] [PubMed] [Google Scholar]
  20. Zeldin M. H., Schiff J. A. RNA metabolism during light-induced chloroplast development in euglena. Plant Physiol. 1967 Jul;42(7):922–932. doi: 10.1104/pp.42.7.922. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES