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. 1974 Apr;53(4):562–568. doi: 10.1104/pp.53.4.562

Relationship of Ribonucleic Acid Metabolism in Embryo and Aleurone to α-Amylase Synthesis in Barley 1,2

Henri A Van Onckelen a,3, Rita Verbeek a,3, Anwar A Khan a
PMCID: PMC541397  PMID: 16658743

Abstract

RNA metabolism of embryo and aleurone of barley grains (Hordeum vulgare L. cv. Himalaya) was studied to elucidate the role of these tissues in the control of α-amylase synthesis and germination. The extent of 3H-uridine incorporated into various RNA classes of the embryo during the first 12 hours of germination was low but constant. Subsequently, there was a rapid increase in RNA synthesis of all fractions. In the aleurones, after 16 hours, a gradual decrease in 3H-uridine incorporation was observed, and by the time the synthesis of RNA in the aleurones had stopped, α-amylase level was at its highest in the grain.

On transfer to accelerated aging conditions (43 C; 85% relative humidity), the grains lost their viability within 4 weeks. That this was due to a rapid deterioration of the embryo and not of the aleurone was apparent in studies on α-amylase formation, RNA metabolism, and ATP content in grains in various physiological states reported here. Results presented here also reveal a marked influence of the embryo and GA3 on the quality of the newly synthesized RNAs. Aleurones which lacked the impulse of embryo or GA3 were capable of synthesizing RNA but these RNAs were less heterodisperse than RNAs from aleurones which were under the influence of an embryo or GA3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIGGS D. E. Simplification of the assay of enzymes using non-linear reaction-progress graphs. Enzymologia. 1962 Feb 15;24:97–104. [PubMed] [Google Scholar]
  2. Chandra G. R., Varner J. E. Gibberellic acid-controlled metabolism of RNA in aleurone cells of barley. Biochim Biophys Acta. 1965 Dec 9;108(4):583–592. doi: 10.1016/0005-2787(65)90055-9. [DOI] [PubMed] [Google Scholar]
  3. Ching T. M. Adenosine triphosphate content and seed vigor. Plant Physiol. 1973 Feb;51(2):400–402. doi: 10.1104/pp.51.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cleland R., McCombs N. Gibberellic acid: action in barley endosperm does not require endogenous auxin. Science. 1965 Oct 22;150(3695):497–498. doi: 10.1126/science.150.3695.497. [DOI] [PubMed] [Google Scholar]
  5. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johri M. M., Varner J. E. Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid. Proc Natl Acad Sci U S A. 1968 Jan;59(1):269–276. doi: 10.1073/pnas.59.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khan A. A., Andersen L., Gaspar T. Abscisic Acid-induced Changes in Nucleotide Composition of Rapidly Labeled Ribonucleic Acid Species of Lentil Root: Reversal by Kinetin. Plant Physiol. 1970 Sep;46(3):494–495. doi: 10.1104/pp.46.3.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Khan A. A. Cytokinins: permissive role in seed germination. Science. 1971 Mar 5;171(3974):853–859. doi: 10.1126/science.171.3974.853. [DOI] [PubMed] [Google Scholar]
  9. Khan A. A., Verbeek R., Waters E. C., van Onckelen H. A. Embryoless Wheat Grain: A Natural System for the Study of Gibberellin-induced Enzyme Formation. Plant Physiol. 1973 Apr;51(4):641–645. doi: 10.1104/pp.51.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. St John J. B. Determination of ATP in Chlorella with the luciferin-luciferase enzyme system. Anal Biochem. 1970 Oct;37(2):409–416. doi: 10.1016/0003-2697(70)90066-7. [DOI] [PubMed] [Google Scholar]
  11. Varner J. E. Gibberellic Acid Controlled Synthesis of alpha-Amylase in Barley Endosperm. Plant Physiol. 1964 May;39(3):413–415. doi: 10.1104/pp.39.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zwar J. A., Jacobsen J. V. A Correlation between a Ribonucleic Acid Fraction Selectively Labeled in the Presence of Gibberellic Acid and Amylase Synthesis in Barley Aleurone Layers. Plant Physiol. 1972 Jun;49(6):1000–1006. doi: 10.1104/pp.49.6.1000. [DOI] [PMC free article] [PubMed] [Google Scholar]

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